New pair of primers having specified nucleotide sequences to introduce a mutation into the nucleotide sequence encoding the potato translation initiation factor eIF4E1 using site-directed mutagenesis
	LEBEDEVA M V; NIKONOV O S; NIKONOVA E I; BABAKOV A V; ZLOBIN N E; TARANOV V V
	2023-03-14
专利权人	SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard)
申请日期	2023-03-14
专利号	RU2023105946-A
成果简介	NOVELTY - Pair of primers having specified nucleotide sequences to introduce a mutation into the nucleotide sequence encoding the potato translation initiation factor eIF4E1 using site-directed mutagenesis, as a result of which the ttt triplet is replaced by a ctt triplet, which leads to a change in the amino acid at position 66 from phenylalanine to leucine, is new. USE - Used as pair of primers. ADVANTAGE - None given. DETAILED DESCRIPTION - Pair of primers 5'-aatagggctatcaagccaaaaagtcca-3' and 5'-tggactttttggcttgatagccctatt-3' to introduce a mutation into the nucleotide sequence encoding the potato translation initiation factor eIF4E1 using site-directed mutagenesis, as a result of which the ttt triplet is replaced by a ctt triplet, which leads to a change in the amino acid at position 66 from phenylalanine to leucine, is new An INDEPENDENT CLAIM is also included for a nucleic acid molecule encoding a mutant translation initiation factor eIF4E1 of potato with a substitution of phenylalanine for leucine in the amino acid sequence fragment Trp-Phe-Asp-Ser-Pro-Ile-Ala-Lys-Ser-Arg-Gln-Thr-Ala-Trp-Gly-Ser-Ser-Leu-Arg-Asn-Val-Tyr-Thr or in an identical one, not less than 80% in any variety of cultivated potato or other plant.
IPC 分类号	A01H-006/82 ; C12N-015/29
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/22176
专题	中国科学院新疆生态与地理研究所
作者单位	SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard)
推荐引用方式 GB/T 7714	LEBEDEVA M V,NIKONOV O S,NIKONOVA E I,et al. New pair of primers having specified nucleotide sequences to introduce a mutation into the nucleotide sequence encoding the potato translation initiation factor eIF4E1 using site-directed mutagenesis. RU2023105946-A[P]. 2023.

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