New primer pair for introducing mutation into nucleotide sequence encoding potato translation initiation factor eIF4E1 using site-directed mutagenesis
	LEBEDEVA M V; NIKONOV O S; NIKONOVA E I; BABAKOV A V; ZLOBIN N E; TARANOV V V
	2023-03-14
专利权人	FEDERAL STATE BUDGETARY INST (FSBI-C)
申请日期	2023-03-14
专利号	RU2023105947-A
成果简介	NOVELTY - A primer pair for introducing a mutation into the nucleotide sequence encoding the potato translation initiation factor eIF4E1 using site-directed mutagenesis, resulting in the CCT triplet being replaced by a TCT triplet and which leads to change in the amino acid at position 69 from proline to serine, is new. USE - The primer pair is useful for introducing mutation into nucleotide sequence for producing a functional translation initiation factor eIF4E1 of potato with a p69s mutation. ADVANTAGE - The primer pair produces functional translation initiation factor eIF4E1 of potato (Solanum tuberosum) with a p69s mutation that disrupts its interaction with a wide spectrum of viral genome-linked protein (VPg) variants of potato virus Y. DETAILED DESCRIPTION - A primer pair comprising 5'-tggtttgatagctctattgctaaatc-3' (SEQ ID NO: Not defined) and 5'-gatttagcaatagagctatcaaacca-3' (SEQ ID NO: Not defined) for introducing a mutation into the nucleotide sequence encoding the potato translation initiation factor eIF4E1 using site-directed mutagenesis, resulting in the CCT triplet being replaced by a TCT triplet leading to change in the amino acid at position 69 from proline to serine, is new. An INDEPENDENT CLAIM is included for a nucleic acid molecule, which encodes a mutant translation initiation factor eIF4E1 of potato with a proline to serine substitution in the amino acid sequence fragment comprising an 23 amino acid sequence (SEQ ID NO: Not defined) fully defined in the specification or a sequence exhibiting at least 80% identity to the above sequence in any variety of cultivated potato or other plant.
IPC 分类号	A01H-006/82 ; C12N-015/29
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/22175
专题	中国科学院新疆生态与地理研究所
作者单位	FEDERAL STATE BUDGETARY INST (FSBI-C)
推荐引用方式 GB/T 7714	LEBEDEVA M V,NIKONOV O S,NIKONOVA E I,et al. New primer pair for introducing mutation into nucleotide sequence encoding potato translation initiation factor eIF4E1 using site-directed mutagenesis. RU2023105947-A[P]. 2023.

条目包含的文件
条目无相关文件。

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。