Obtaining extracellular phenoloxidase enzymes by strain of fungus Fomes fomentarius, by cultivating fungus on optimized nutrient medium with addition of sawdust extract, separating resulting culture liquid from mycelium
	MATUSEVICH S V; KHALIMOVA L KH; PETUKHOVA N I; ZORIN V V
	2023-03-24
专利权人	MATUSEVICH S V (MATU-Individual)
申请日期	2023-03-24
专利号	RU2023107120-A
成果简介	NOVELTY - Obtaining extracellular phenoloxidase enzymes by the strain of the fungus Fomes fomentarius VKPM F-1531, comprises cultivating fungus on an optimized nutrient medium with the addition of 30 vol.% sawdust extract at an initial pH of 5, a temperature of 25-30° C, excess pressure in the apparatus of 2.2 atmosphere, aeration of 1/*1-1/minute, mechanical stirring of the medium at 200 rotation per minute for 8 days, separating the resulting culture liquid from the mycelium by centrifugation at 10000 rotation per minute for 7-8 minutes and as a result an enzyme preparation is obtained with a maximum activity of 17-20 units/ml (for pyrocatechol) or 770-830 units/ml (for 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid). USE - The method is useful for obtaining extracellular phenoloxidase enzymes by the strain of the fungus Fomes fomentariusVKPM F-1531. ADVANTAGE - None given.
IPC 分类号	A01G-018/00 ; A01G-018/20 ; C12N-009/02
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/22126
专题	中国科学院新疆生态与地理研究所
作者单位	MATUSEVICH S V (MATU-Individual)
推荐引用方式 GB/T 7714	MATUSEVICH S V,KHALIMOVA L KH,PETUKHOVA N I,et al. Obtaining extracellular phenoloxidase enzymes by strain of fungus Fomes fomentarius, by cultivating fungus on optimized nutrient medium with addition of sawdust extract, separating resulting culture liquid from mycelium. RU2023107120-A[P]. 2023.

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