| Detecting neutrophil extracellular traps (NETs) and cytological changes associated with NETosis in supravitally stained oral fluid preparation involves e.g. diluting oral fluid, visualizing results, and calculating percentage of NETs | |
| 2023-04-11 | |
| 专利权人 | FEDERAL STATE BUDGETARY EDUCATIONAL (BUDG-Non-standard) |
| 申请日期 | 2023-04-11 |
| 专利号 | RU2023109076-A; RU2832060-C2 |
| 成果简介 | NOVELTY - Detecting and assessing neutrophil extracellular traps (NETs) and cytological changes associated with NETosis in a supravitally stained oral fluid preparation, comprises (a) collecting oral fluid and diluting with a phosphate buffer solution, adding propidium iodide and monoclonal antibodies to cluster of differentiation (CD)45 labeled with fluorescein isothiocyanate, previously diluted with a phosphate buffer solution and incubating under a cover glass in the dark, (b) visualizing the results by fluorescence microscopy using light filters, (c) calculating the percentage of NETs of different types, and (d) additionally calculating the coefficient of capture of microorganisms by neutrophil traps. USE - The method is useful for detecting and assessing NETs and cytological changes associated with NETosis in a supravitally stained oral fluid preparation. ADVANTAGE - None given. |
| IPC 分类号 | A61B-005/00 ; G01N-001/30 ; G01N-033/569 ; G01N-033/577 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/22022 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY EDUCATIONAL (BUDG-Non-standard) |
| 推荐引用方式 GB/T 7714 | ZOLOTOV A N,NOVIKOV D G,ZOLOTOVA L I,et al. Detecting neutrophil extracellular traps (NETs) and cytological changes associated with NETosis in supravitally stained oral fluid preparation involves e.g. diluting oral fluid, visualizing results, and calculating percentage of NETs. RU2023109076-A; RU2832060-C2[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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