Universal cellular test system used to analyze antiinflammatory activity of substances, has 96-well plate, suspension of profile cells having ability to stimulate inflammation trigger, culture medium, and priming medium to sensitize cells
	VOLOVA L T; ASINA N K
	2023-05-03
专利权人	UNIV SAMARA MEDICAL (USMR-C)
申请日期	2023-05-03
专利号	RU2023111428-A
成果简介	NOVELTY - Universal cellular test system for analyzing the antiinflammatory activity of substances, comprises a 96-well plate, a suspension of profile cells that have the ability to dose-dependently stimulate an inflammation trigger and are obtained from target tissues involved in the pathogenesis of the disease, a culture medium, a priming medium, and an inflammation trigger, where the suspension of cells is seeded into the plate and cultured, the priming medium is added to sensitize the cells, the study of the cells is continued in 3 types of reaction media i.e. control, which is a pure culture medium, a stimulated culture medium with the addition of an inflammation trigger, and neutralized culture media with an inflammation trigger and its inhibitor. A method for using universal cellular test system, comprises studying the level of production of inflammation mediator molecules by cells after their incubation with inflammatory trigger, mixture of inflammation trigger and substance under study. USE - The universal cellular test system is useful for analyzing the antiinflammatory activity of substances (claimed). ADVANTAGE - None given. DETAILED DESCRIPTION - Universal cellular test system for analyzing the antiinflammatory activity of substances, comprises a 96-well plate, a suspension of profile cells that have the ability to dose-dependently stimulate an inflammation trigger and are obtained from target tissues involved in the pathogenesis of the disease, a culture medium, a priming medium, and an inflammation trigger, where the suspension of cells is seeded into the plate and cultured, the priming medium is added to sensitize the cells, the study of the cells is continued in 3 types of reaction media i.e. control, which is a pure culture medium, a stimulated culture medium with the addition of an inflammation trigger, and neutralized culture media with an inflammation trigger and it's inhibitor. A method for using universal cellular test system for analyzing the antiinflammatory activity of substances, comprises studying the level of production of inflammation mediator molecules by cells after their incubation with an inflammatory trigger, a mixture of an inflammation trigger and a substance under study, where the substance under study in different concentrations can incubate, collect the media from the wells of the plate, determine and compare the level of production of inflammation mediator molecules by the cells, thus assessing the antiinflammatory activity of the substance under study.
IPC 分类号	C12N-005/00 ; G01N-033/48
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21922
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV SAMARA MEDICAL (USMR-C)
推荐引用方式 GB/T 7714	VOLOVA L T,ASINA N K. Universal cellular test system used to analyze antiinflammatory activity of substances, has 96-well plate, suspension of profile cells having ability to stimulate inflammation trigger, culture medium, and priming medium to sensitize cells. RU2023111428-A[P]. 2023.

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