Detecting volatile organic metabolites in crops for early detection of blight disease, involves collecting volatile organic metabolite from leaf or rice, accumulating metabolites in chamber, detecting biogenic volatile organic compounds using 2-terminal chemiresistor, and identifying blight disease
	SINGH S P; SIRCAR D; WAGHMODE B; KIRAN R M; YADAV Y
	2023-05-12
专利权人	INDIAN COUNCIL AGRIC RES (ICOU-C)
申请日期	2023-05-12
专利号	IN202311033681-A
成果简介	NOVELTY - Method for detecting volatile organic metabolites in crops infected with blight disease using 2-terminal chemiresistor involves: (i) collecting volatile organic metabolites compounds (VOCs) from the intact crop leaf of rice (Oryzae sativum); (ii) accumulating VOCs for 1-3 hours in the chamber followed by extraction; (iii) detecting biogenic volatile organic compounds using 2-terminal chemiresistor, where when a potential bias of 0.3-1V is applied across the terminals of the resistor, and the current is measured real time, two volatile organic compounds comprising α-ionone and E-2-hexenal cause a reduction in the measured current, resulting in identification of blight disease caused by Xanthomonas oryzae in rice as the decrement in current is proportional to the concentration of VOC's in the vicinity of the chemiresistor. USE - Method for detecting volatile organic metabolites in crops e.g. rice infected with blight disease for early detection of blight disease in rice crops. ADVANTAGE - The method enables detection of volatile organic metabolites using portable and non-invasive sensor in accurate, non-invasive, and cost-effective manner, and monitors early infection of rice. DETAILED DESCRIPTION - Method for detecting volatile organic metabolites in crops infected with blight disease using 2-terminal chemiresistor involves: (i) collecting VOCs from the intact crop leaf of rice (Oryzae sativum); (ii) accumulating VOCs for 1-3 hours in the chamber followed by extraction; (iii) detecting biogenic volatile organic compounds using 2-terminal chemiresistor, where when a potential bias of 0.3-1V is applied across the terminals of the resistor, and the current is measured real time, two volatile organic compounds comprising α-ionone and E-2-hexenal cause a reduction in the measured current, resulting in identification of blight disease caused by Xanthomonas oryzae in rice as the decrement in current is proportional to the concentration of VOC's in the vicinity of the chemiresistor; or detecting volatile organic metabolites in crops infected with blight disease using 3-terminal transistor involves: (a) collecting VOCs from the intact crop leaf; (b) placing a single leaf inside the collection chamber followed by sealing the base of the leaf by paraffin; (c) accumulating VOCs for 1-3 hours; (d) subjecting the VOC's to 3-terminal transistor operated in the saturation regime with 1 V potential bias applied at the drain terminal (Vds) and 1V potential bias applied at the gate terminal (Vgs) with respect to the source terminal; and (e) measuring the current between the source and the drain terminals of the 3-terminal transistor, where the measured current decreases with increased concentration of the biomarkers, in the environment around the sensing element, resulting in identification of blight disease caused by Xanthomonas oryzae in rice as the decrement in current is proportional to the concentration of the VOC's in the vicinity of the 3-terminal transistor.
IPC 分类号	B01D-053/86 ; B82Y-015/00 ; G01N-027/12 ; G01N-033/00 ; G01N-033/22
国家	印度
专业领域	农业科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21872
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN COUNCIL AGRIC RES (ICOU-C)
推荐引用方式 GB/T 7714	SINGH S P,SIRCAR D,WAGHMODE B,et al. Detecting volatile organic metabolites in crops for early detection of blight disease, involves collecting volatile organic metabolite from leaf or rice, accumulating metabolites in chamber, detecting biogenic volatile organic compounds using 2-terminal chemiresistor, and identifying blight disease. IN202311033681-A[P]. 2023.

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