Isolating nucleic acids from sectional material by treating biomaterial sample with denaturing buffer solution, incubating, centrifuging, adding isopropyl alcohol to supernatant, vortexing, washing, dissolving in deionized water, and storing
	MARKARYAN A YU; BOLGAROVA E V; CHERNYSHEVA A E; KOROTKOVA I A; SEMENOV A V
	2023-06-05
专利权人	VIROM FEDERAL SERVICE SURVEILLANCE CONSU (VIRO-Non-standard)
申请日期	2023-06-05
专利号	RU2023114719-A
成果简介	NOVELTY - Method for isolating nucleic acids from sectional material involves performing mechanical processing of the biomaterial, treating the biomaterial sample with a 400 μ l denaturing buffer solution comprising 0.1 M tris-hydrochloric acid, 0.1 M EDTA, 0.5 M sodium chloride, 1% sodium dodecyl sulfate (SDS) and 20 μ l proteinase K solution at a concentration of 20 mg/ml, incubating the mixture for 5-6 hours and precipitating non-target biopolymers and cellular debris by centrifugation in a 5 M sodium chloride saline solution with a volume of 500 μ l at a speed of 10000-15000 g, taking an aliquot of 400 μ l from the upper layer and transferring to a new tube, adding 400 μ l isopropyl alcohol to the collected supernatant, vortexing and centrifuging for 10 minutes at 10000 g, removing the supernatant, washing the sediment twice and centrifuging with 75% ethanol, dissolving the sediment containing nucleic acids in deionized water, and storing at -20° C. USE - The method is useful for isolating nucleic acids from sectional material.
IPC 分类号	C12N-015/10 ; C12P-019/34 ; G01N-033/48
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21696
专题	中国科学院新疆生态与地理研究所
作者单位	VIROM FEDERAL SERVICE SURVEILLANCE CONSU (VIRO-Non-standard)
推荐引用方式 GB/T 7714	MARKARYAN A YU,BOLGAROVA E V,CHERNYSHEVA A E,et al. Isolating nucleic acids from sectional material by treating biomaterial sample with denaturing buffer solution, incubating, centrifuging, adding isopropyl alcohol to supernatant, vortexing, washing, dissolving in deionized water, and storing. RU2023114719-A[P]. 2023.

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