| METHOD FOR MONITORING ACTIVITY OF SINGLE ENZYME MOLECULES | |
| 2023-06-12 | |
| 专利权人 | SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard) |
| 申请日期 | 2023-06-12 |
| 专利号 | RU2827870-C1 |
| 成果简介 | NOVELTY - Invention relates to enzymology of single molecules and can be used to study enzymatic activities of single enzyme molecules using nanotechnology, as well as processes of denaturation of single enzyme molecules. Method for monitoring the activity of single molecules of the enzyme of the class of oxidoreductases by the example of horseradish peroxidase and the class of hydrolases by the example of asparaginase is carried out using a nanopore detector. Nanopore detector comprises a cuvette with cis- and trans-chambers, between which there is a nano-pore in the form of a cone made by the electron-beamdrilling (EBD) method in Six-Ny. Method involves introducing a buffer solution containing an enzyme into a cis chamber of the cuvette, and a buffer solution without an enzyme into the trans chamber. Voltage is supplied between cis- and trans-chambers, with the help of which a single enzyme molecule is introduced into the nanopore with its subsequent immobilization. Immobilisation process is controlled by the change in the electric current flowing through the nanopore. Cis chamber is washed from non-immobilized enzyme molecules. Buffer solution of a substrate inducing enzyme activity is added to the cis-chamber. Change in the current flowing through the nanopore is used to monitor the activity of the enzyme during interaction of its molecule with the molecule of the introduced buffer solution of the substrate. USE - Nanotechnology. ADVANTAGE - Group of inventions provides a wider range of technical means for realizing the method of monitoring activity of single molecules. 4 cl, 3 dwg, 3 ex |
| IPC 分类号 | C12M-001/00 ; C12M-001/34 ; C12Q-001/25 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/21643 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | SUPERHARD & NEW CARBON MATERIALS INST (SUPE-Non-standard) |
| 推荐引用方式 GB/T 7714 | IVANOV YU D,ABLEEV A N,BUKATIN A S,et al. METHOD FOR MONITORING ACTIVITY OF SINGLE ENZYME MOLECULES. RU2827870-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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