Composition for gene specific amplification and detection of carbapenem resistance genes, comprises deoxynucleoside triphosphates, magnesium chloride, Taq polymerase, ethylenediaminetetraacetic acid buffer, ultrapure water, bacterial DNA (template), and forward and reverse primers
	DUDANI H; HUSAIN A A; KASHYAP R S
	2023-06-29
专利权人	KASHYAP R S (KASH-Individual)
申请日期	2023-06-29
专利号	IN202221076711-A
成果简介	NOVELTY - Composition comprises Deoxynucleoside triphosphates (dNTPs), magnesium chloride, Taq polymerase, Ethylenediaminetetraacetic acid (EDTA) buffer, ultrapure water, the bacterial DNA (template) and primers; where the primers comprise forward primers and reverse primers, each primer being added in an amount of 4 vol.%; where the ratio of forward primers: reverse primers is 1:1. USE - Composition for gene specific amplification and detection of carbapenem resistance genes. ADVANTAGE - The method is rapid and accurate. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for an in-vitro method for genotypic detection of carbapenem resistance genes using a heat-based gene amplification reaction which involves: (a) isolating the bacterial DNA containing the specific genes associated with carbapenem resistance; (b) preparing a mixture by adding Taq polymerase, dNTPs, magnesium chloride, EDTA buffer, ultrapure water and pairs of carbapenem resistance gene specific primers to the isolated DNA as obtained; (c) subjecting the product as obtained for amplification by heating the product at 95 ℃ for 5 minutes followed by annealing 35 cycles and extended heat treatment at 72 ℃ for 8 minute; and (iv) subjecting the product as obtained for a gel-based detection.
IPC 分类号	C04B-028/32 ; C07K-014/195 ; C07K-014/415 ; C12N-009/12 ; C12Q-001/689
国家	印度
专业领域	材料科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21459
专题	中国科学院新疆生态与地理研究所
作者单位	KASHYAP R S (KASH-Individual)
推荐引用方式 GB/T 7714	DUDANI H,HUSAIN A A,KASHYAP R S. Composition for gene specific amplification and detection of carbapenem resistance genes, comprises deoxynucleoside triphosphates, magnesium chloride, Taq polymerase, ethylenediaminetetraacetic acid buffer, ultrapure water, bacterial DNA (template), and forward and reverse primers. IN202221076711-A[P]. 2023.

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