Preparing antibacterial activity and osteogenic potential exhibiting insulin-like growth factor-1 loaded anionic poly (lactic-co-glycolic acid) nanoparticles, comprises preparing organic phase, and aqueous phase, preparing water in oil in water emulsion, and preparing nanoparticles, and storing
	KANDASAMY R; KRISHNASWAMI V; VIJAYARAGHAVALU S; VAIDYANATHAN A K; RAZA F B
	2023-07-12
专利权人	SRI RAMACHANDRA HIGHER EDUCATION & RES (SRIR-Non-standard)
申请日期	2023-07-12
专利号	IN202341046964-A
成果简介	NOVELTY - Preparing antibacterial activity and osteogenic potential exhibiting insulin-like growth factor-1 (IGF-1) loaded anionic poly (lactic-co-glycolic acid) (PLGA) nanoparticles with enhanced encapsulation efficiency and sustained release of charged growth factor comprises: (a) preparing organic phase including dissolving 100 mg PLGA in acetone, and stirring to form organic phase; (b) preparing aqueous phase including dispensing Tween 80(RTM: Polyoxyethylene (20) sorbitan monooleate) in water, and stirring to form aqueous phase; (c) preparing water in oil in water emulsion including adding aqueous phase into the organic phase dropwise, and performing sonication using probe sonicator; and (d) preparing IGF-1 loaded anionic PLGA nanoparticles by stirring water-in-oil emulsion, placing in vacuum desiccator, centrifuging to form pellet I and supernatant I, and adding pellet II to sucrose solution, and lyophilizing to form IGF-1 loaded anionic PLGA nanoparticles and storing. USE - Method for preparing antibacterial activity and osteogenic potential exhibiting IGF-1 loaded anionic PLGA nanoparticles with enhanced encapsulation efficiency and sustained release of charged growth factor. No biological data given. ADVANTAGE - The method exhibits biodegradable nanoparticles with sufficient encapsulation efficiency, sustained release and less toxic, controls oral biofilm formation and enhances peri-implant osteogenesis, and has more stability and target efficiency. DETAILED DESCRIPTION - Preparing antibacterial activity and osteogenic potential exhibiting insulin-like growth factor-1 (IGF-1) loaded anionic poly (lactic-co-glycolic acid) (PLGA) nanoparticles with enhanced encapsulation efficiency and sustained release of charged growth factor comprises: (a) preparing organic phase including dissolving 100 mg PLGA in 3-5 ml acetone, and adding 100 µg in 200 µl/MiliQ(RTM: Water purification system) water of IGF-1 and stirring on magnetic stir plate at 6000 revolutions per minute (rpm) for 1 hour in covered container closed with aluminium foil to form organic phase; (b) preparing aqueous phase including dispensing 0.01% Tween 80(RTM: Polyoxyethylene (20) sorbitan monooleate) in 18 ml water, and stirring on magnetic stir plate at 600 rpm for 1 hour in covered container closed with aluminium foil to form aqueous phase; (c) preparing water in oil in water emulsion including adding aqueous phase into the organic phase dropwise, and performing sonication using probe sonicator at 40% on ice bath to maintain 4℃, at 2 minutes intervals for 3 times to form water-in-oil-in-water emulsion; and (d) preparing IGF-1 loaded anionic PLGA nanoparticles by stirring water-in-oil emulsion for 24 hours at room temperature on magnetic stir plate at 600 rpm with face velocity of 200 fpm in face velocity of 200 feet per minute (fpm) in a fume hood in covered container closed with aluminium foil with holes, placing in vacuum desiccator, magnetic stirring at 200 rpm for 1-2 hour to evaporate organic solvent, and centrifuging 3× at 20000 rpm for 20 minutes to form pellet I and supernatant I, suspending the pellet I with 50 ml MiliQ(RTM: Water purification system) water in a falcon's tube, centrifuging in cooling centrifuge (2x) at 1000 rpm for 10 minutes to form pellet II and supernatant II and adding pellet II to 5 ml sucrose solution in falcon's tube covered with paraffin film with holes, placing in freezer at -80℃ for 3-4 hrs and freeze-drying in lyophilizer, and lyophilizing for 48 hrs to form IGF-1 loaded anionic PLGA nanoparticles and storing at -20℃, where the obtained supernatant I and II are discarded. An INDEPENDENT CLAIM is included for an antibacterial activity and osteogenic potential exhibiting IGF-1 loaded anionic PLGA nanoparticles with enhanced encapsulation efficiency and sustained release of charged growth factor prepared by the method.
IPC 分类号	A61K-038/30 ; A61K-009/16 ; A61K-009/51 ; A61L-027/54 ; B01J-035/10
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21325
专题	中国科学院新疆生态与地理研究所
作者单位	SRI RAMACHANDRA HIGHER EDUCATION & RES (SRIR-Non-standard)
推荐引用方式 GB/T 7714	KANDASAMY R,KRISHNASWAMI V,VIJAYARAGHAVALU S,et al. Preparing antibacterial activity and osteogenic potential exhibiting insulin-like growth factor-1 loaded anionic poly (lactic-co-glycolic acid) nanoparticles, comprises preparing organic phase, and aqueous phase, preparing water in oil in water emulsion, and preparing nanoparticles, and storing. IN202341046964-A[P]. 2023.

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