| METHOD FOR DETECTING CLOSTRIDIUM SPECIES CLOSTRIDIUM SPOROGENES, CLOSTRIDIUM PERFRINGENS AND CLOSTRIDIUM SORDELLII | |
| 2023-07-14 | |
| 专利权人 | AS RUSSIA SIBERIAN FEDERAL RES CENT AGRO (ASSI-Non-standard) |
| 申请日期 | 2023-07-14 |
| 专利号 | RU2826158-C1 |
| 成果简介 | NOVELTY - Described is a method for detecting Clostridium sporogenes, Clostridium perfringens and Clostridium sordellii, including preliminary enrichment on selective nutrient media and multiplex polymerase chain reaction in real time. Method is characterized by that detection of Clostridium sporogenes is carried out by amplification of gerKA gene section using developed primers and probe, detection of Clostridium perfringens is carried out by amplification of the plc gene section using the developed primers and a probe, detection of the Clostridium sordellii species is carried out by amplification of a portion of the NanS gene using the developed primers and a probe. USE - Biotechnology. ADVANTAGE - Development of highly specific and sensitive method based on multiplex polymerase chain reaction (PCR) in real time, enabling simultaneous detection of Clostridium sporogenes, Clostridium perfringens and Clostridium sordellii in samples of pathological material from sick animals, as well as in mixed and pure bacterial cultures. 1 cl, 5 tbl, 4 ex |
| IPC 分类号 | C12Q-001/68 ; C12Q-001/6844 ; C12Q-001/6876 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/21298 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | AS RUSSIA SIBERIAN FEDERAL RES CENT AGRO (ASSI-Non-standard) |
| 推荐引用方式 GB/T 7714 | NEFEDCHENKO A V,GLOTOVA T I,SUDORGINA T E,et al. METHOD FOR DETECTING CLOSTRIDIUM SPECIES CLOSTRIDIUM SPOROGENES, CLOSTRIDIUM PERFRINGENS AND CLOSTRIDIUM SORDELLII. RU2826158-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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