Spiral microfluidic device for separating target white blood cells (WBCs) from blood sample, has inlet reservoir into which blood sample is loaded, and main channel originating from inlet reservoir comprising single spiral and having first bifurcation and second bifurcation along spiral length
	SIDDHARTHA T; SUNIL B; VADIRAJ H; JACOB P; PANDURANG M S
	2023-07-18
专利权人	BITS BIOCYTIH FOUND (BITS-Non-standard) ; BIRLA INST TECHNOLOGY & SCI PILANI (BIRL-Non-standard)
申请日期	2023-07-18
专利号	IN202311048220-A
成果简介	NOVELTY - The spiral microfluidic device for separating target white blood cells (WBCs) from a blood sample, has: an inlet reservoir (1) into which the blood sample is loaded; and a main channel (2) originating from the inlet reservoir comprising a single spiral (2.3) and having a first bifurcation (2.4) and second bifurcation (2.5) along the spiral length and branching from the outer wall. The first bifurcation has a branching channel, tangential to the main channel, to divert non-target blood cells into an outlet (3), while the WBCs continue to move along the inner wall of the main channel. The second bifurcation is located towards the end of the main channel, has a branching channel to an outlet (4) for removing non-target cells, while the purer WBCs continue to move along the inner wall of the main channel for collection at a WBC outlet (5). USE - Spiral microfluidic device for separating target WBCs from a blood sample, where the purified WBC is useful in biological assays. ADVANTAGE - The device utilizes inertial microfluidics to separate WBCs from other blood components with high purity. The combination of the factors results in a higher-purity separation of WBCs from other blood cells such as red blood cells and platelets providing a sample that can be used for biological assays. The single curve and the first bifurcation along the length of the spiral channel design are specifications that enable the microdevice to separate WBCs in a single cycle with high processing speed. The use of a single curve microchannel consumes a low amount of the blood sample and is easy to operate. The single spiral design reduces manufacturing costs and complexity. This microdevice takes up much less space and reduces the probability of failure during operation. It also operates at a higher hematocrit of diluted blood than current microdevices without requiring any recirculation or additional reagents. These features reduce the steps and complexity associated with further dilution, recirculation, or use of agents while offering reasonably similar concentration and purity of WBCs at the output. The device separates and isolates WBCs with high effectiveness (minimum presence of red blood cells) using small quantities of blood obtained from finger-prick method. This microdevice does not require any external force fields and WBC labelling for separation. Due to its small size, the microdevice can be easily transported to the patient's location, and when combined with a biosensor, it can provide immediate on-site diagnostics. The device's simplicity makes it easier to handle for pathologists than existing complex microdevices and conventional techniques of WBC separation. DESCRIPTION OF DRAWING(S) - The drawing shows the microfluidic device for separation of WBCs from blood. 1Inlet reservoir 2Main channel 2.3Single spiral 2.4First bifurcation 2.5Second bifurcation 3,4,5Outlets
IPC 分类号	B01L-003/00 ; G01N-015/00 ; G01N-015/10 ; G01N-015/14 ; G01N-033/49
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21255
专题	中国科学院新疆生态与地理研究所
作者单位	1.BITS BIOCYTIH FOUND (BITS-Non-standard) 2.BIRLA INST TECHNOLOGY & SCI PILANI (BIRL-Non-standard)
推荐引用方式 GB/T 7714	SIDDHARTHA T,SUNIL B,VADIRAJ H,et al. Spiral microfluidic device for separating target white blood cells (WBCs) from blood sample, has inlet reservoir into which blood sample is loaded, and main channel originating from inlet reservoir comprising single spiral and having first bifurcation and second bifurcation along spiral length. IN202311048220-A[P]. 2023.

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