Analyzing antibacterial activity of plant Euphorbia hirta, comprises collecting plant parts, mixing with solvents, screening extracts for phytochemicals to determine total phenolic and flavonoidal content, standardizing, evaluating extracts for antibacterial activity on Staphylococcus aureus
	PUNDIR S; SHARMA N
	2023-07-20
专利权人	UNIV SHOOLINI BIOTECHNOLOGY & MANAGEMENT (USLN-C)
申请日期	2023-07-20
专利号	IN202311048914-A
成果简介	NOVELTY - Analyzing antibacterial activity of plant Euphorbia hirta, comprises: (a) collecting one or more plant parts of the plant, where the one or more parts of the plant are mixed with one or more solvents to obtain a number of extracts which include n-hexane extract, chloroform extract, ethyl acetate extract and ethanol extract; (b) screening the number of extracts for one or more phytochemicals to determine total phenolic and flavonoidal content, where the ethanolic extract is having more phenolic content than the flavonoid content; (c) standardizing the number of extracts by quantification of standard bioactive compounds, where the number of extracts is standardized by using one or more solutions which include ellagic acid, gallic acid, quercetin and rutin, and the factor of retention (Rf) values obtained are 0.47, 0.70, 0.96 and 0.53; and (d) evaluating the number of extracts for antibacterial activity on Staphylococcus aureus. USE - Method for analyzing antibacterial activity of plant Euphorbia hirta. ADVANTAGE - The method needs the least chemicals for analyzing antibacterial activity of the extract, and is cost effective and less time consuming. DETAILED DESCRIPTION - Analyzing antibacterial activity of plant Euphorbia hirta, comprises: (a) collecting one or more plant parts of the plant, where the one or more parts of the plant are mixed with one or more solvents to obtain a number of extracts which include n-hexane extract, chloroform extract, ethyl acetate extract and ethanol extract; (b) screening the number of extracts for one or more phytochemicals to determine total phenolic and flavonoidal content, where the ethanolic extract is having more phenolic content than the flavonoid content; (c) standardizing the number of extracts by quantification of standard bioactive compounds, where the number of extracts is standardized by using one or more solutions which include ellagic acid, gallic acid, quercetin and rutin, and the factor of retention (Rf) values obtained are 0.47, 0.70, 0.96 and 0.53; and (d) evaluating the number of extracts for antibacterial activity on Staphylococcus aureus, where the number of extracts having the ethanolic extract with a zone of inhibition 17.0 mm and having minimum inhibitory concentration (MIC) of 0.25 mg/ml.
IPC 分类号	A61K-036/185 ; A61K-036/47 ; A61K-008/9789 ; A61K-008/9794 ; A61K-009/00
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21219
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV SHOOLINI BIOTECHNOLOGY & MANAGEMENT (USLN-C)
推荐引用方式 GB/T 7714	PUNDIR S,SHARMA N. Analyzing antibacterial activity of plant Euphorbia hirta, comprises collecting plant parts, mixing with solvents, screening extracts for phytochemicals to determine total phenolic and flavonoidal content, standardizing, evaluating extracts for antibacterial activity on Staphylococcus aureus. IN202311048914-A[P]. 2023.

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