Culture medium composition used in kit for in-vitro development of preantral follicles and release of oocytes, comprises Dulbecco's modified eagle medium, lyophilized stromal cell secretomes, and supplements, where supplements are insulin-transferrin-selenium, and epidermal growth factor
	KALTHUR G; JOSHI J M; SEETHARAM R N; NAIR R M T
	2023-07-21
专利权人	MANIPAL ACAD HIGHER EDUCATION (MANI-Non-standard)
申请日期	2023-07-21
专利号	IN202341049385-A
成果简介	NOVELTY - Culture medium composition comprises 50-75 wt.%/vol.% Dulbecco's modified eagle medium (DMEM), 5.0-20.0 wt.%/vol.% lyophilized stromal cell secretomes, and 5.0-20.0 wt.%/vol.% supplements. USE - Culture medium composition used in kit for in-vitro development of preantral follicles and release of oocytes. ADVANTAGE - The method provide a way of in-vitro development of preantral follicles and release of oocytes in a simple and easy manner. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: Kit for in-vitro development of preantral follicles and release of oocytes, which comprises first container comprising lyophilized stromal cell secretomes, second container comprising Dulbecco's modified eagle medium (DMEM), third container comprising one supplement, and a leaflet providing use instruction, where the third container comprises a separate compartment for each of supplements selected from insulin-transferrin-selenium (ITS), epidermal growth factor (EGF), non-essential amino acids (NEAA), bovine serum albumin (BSA), recombinant follicle-stimulating hormone (rFSH), fetal bovine serum (FBS) and human chorionic gonadotrophin (hCG); and Method for in-vitro development of preantral follicles and release of oocytes, which involves: (1) providing human mesenchymal stromal cells from bone marrow (BM), adipose tissue (AT), and Wharton's jelly (WJ); (2) cryopreserving said human mesenchymal stromal cells using a freezing mixture to obtain cryopreserved human mesenchymal stromal cells; (3) subjecting the cryopreserved human mesenchymal stromal cells to thawing and culturing till 70 to 80% confluency in a medium comprising Dulbecco's modified eagle medium (DMEM), followed by centrifugation and filtering to obtain stromal cell secretomes; (4) lyophilizing stem cell secretomes using 4 wt.% Trehalose to obtain lyophilized stem cell secretomes; and (5) culturing pre-antral follicles in a Dulbecco's modified eagle medium (DMEM) enriched with the lyophilized stem cell secretomes and supplementing with bovine serum albumin (BSA), insulin-transferrin-selenium (ITS), epidermal growth factor (EGF), non-essential amino acids (NEAA), recombinant follicle-stimulating hormone (rFSH) and fetal bovine serum (FBS), where the supplementing is carried out in every alternate day for 8-12 days and the recombinant follicle-stimulating hormone (rFSH) is replaced by human chorionic gonadotrophin (hCG) from day 5, where the lyophilized stromal cell secretomes are mixture of bone marrow mesenchymal stromal cell secretome, Wharton's jelly mesenchymal stromal cell secretome, and adipose tissue mesenchymal stromal cell secretome.
IPC 分类号	A61K-035/28 ; A61P-005/00 ; A61P-009/00 ; C12N-005/075 ; C12N-005/0775
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21198
专题	中国科学院新疆生态与地理研究所
作者单位	MANIPAL ACAD HIGHER EDUCATION (MANI-Non-standard)
推荐引用方式 GB/T 7714	KALTHUR G,JOSHI J M,SEETHARAM R N,et al. Culture medium composition used in kit for in-vitro development of preantral follicles and release of oocytes, comprises Dulbecco's modified eagle medium, lyophilized stromal cell secretomes, and supplements, where supplements are insulin-transferrin-selenium, and epidermal growth factor. IN202341049385-A[P]. 2023.

条目包含的文件
条目无相关文件。

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。