Enzymatic formulation used for in situ treatment of agricultural waste, comprises ligninase, cellulase, beta-glucosidase along and an activity enhancer, where activity enhancer is a biosurfactant
	SINGH D; SRIVASTAVA P
	2023-07-21
专利权人	INDIAN TECHNOLOGY VARANASI INST (INTE-Non-standard)
申请日期	2023-07-21
专利号	IN202311049284-A
成果简介	NOVELTY - Enzymatic formulation comprises ligninase, cellulase, β-glucosidase along and an activity enhancer. USE - Enzymatic formulation used for in situ treatment of agricultural waste (claimed). ADVANTAGE - The formulation has 90% degradation efficiency. The biosurfactant enhances the surface activity of enzymes and hydrophilicity of the enzyme mix towards the agricultural biomass. The in situ treatment of agricultural waste in the cropping field is carried out in a simple and easy manner, and it does not require transportation from the field to the site, does not involve any harmful chemicals and thus avoids pollution. The treated agricultural waste is ploughed back in the fields and need not be burned, and complete treatment is achieved within a time span of 10 to 30 days. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for in situ treatment of agricultural waste using the enzymatic formulation, which involves: (1) pre-treatment of the agricultural waste biomass with an alkaline aqueous solution (pH 7.5-8.5); (2) treating with an aqueous buffer solution after 5-7 days of pre-treatment; (3) treating the pre-treated field with the enzymatic formulation twice after 3-5 days of pre-treatment of step (2) by spraying and leaving for 10-12 days; and (4) intermittently treating the field with the enzymatic formulation in every 5 days and ploughing again after 7 days till day 30 for complete treatment.
IPC 分类号	C11D-003/386 ; C12N-015/11 ; C12N-009/42 ; C12Q-001/6883 ; G01N-033/574
国家	印度
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21182
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN TECHNOLOGY VARANASI INST (INTE-Non-standard)
推荐引用方式 GB/T 7714	SINGH D,SRIVASTAVA P. Enzymatic formulation used for in situ treatment of agricultural waste, comprises ligninase, cellulase, beta-glucosidase along and an activity enhancer, where activity enhancer is a biosurfactant. IN202311049284-A[P]. 2023.

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