Preparing chitosan-coated metallic nanoparticle exhibiting antifungal activity, comprises preparing precursor solution by dissolving zinc nitrate in water, adding chitosan solution to precursor solution, adding leaf extract of Lantana camara and Rhamnus triquetra to solution, adding sodium hydroxide
	MANN N A; SHARMA R; THAKUR N; ALTABBAA S
	2023-07-26
专利权人	UNIV SHOOLINI BIOTECHNOLOGY & MANAGEMENT (USLN-C)
申请日期	2023-07-26
专利号	IN202311050383-A
成果简介	NOVELTY - Preparing chitosan-coated metallic nanoparticle comprises: (a) preparing chitosan solution by dissolving 700 mg chitosan in 1% glacial acetic acid; (b) preparing metal precursor solution by dissolving 0.05 M zinc nitrate in 100 mL distilled water, and stirring the mixture; (c) adding 10 mL chitosan solution dropwise to a precursor solution of step (b); (d) heating 15 mL leaf extract obtained from leaves of Lantana camaraand Rhamnus triquetrain water bath; (e) adding leaf extract to the solution of step (a) and magnetically stirring; (f) adding 1M solution of sodium hydroxide to the solution of step (e) and stirring, resulting in a color change of the mixture; (g) leaving obtained suspension undisturbed overnight in a dark environment to obtain precipitate; (h) centrifuging the precipitate at 4000 revolutions/minute to obtain pellet; (i) washing the pellet with distilled water and drying in oven; and (j) transferring dried pellet into a clean bottle for storage. USE - Method for preparing chitosan-coated metallic nanoparticle exhibiting antibacterial and antifungal activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Rosellinia necatrix, and for seed germination and plant growth. Test details are described but no results given. ADVANTAGE - The method provides chitosan-coated metallic nanoparticle having improved stability and biocompatibility with high purity, without using toxic chemicals and solvents, in simple, environmentally friendly and cost-effective manner, and the nanoparticle ensures improved shelf life of cash crops. DETAILED DESCRIPTION - Preparing chitosan-coated metallic nanoparticle comprises: (a) preparing chitosan solution by dissolving 700 mg chitosan in 1% glacial acetic acid; (b) preparing metal precursor solution by dissolving 0.05 M zinc nitrate in 100 mL distilled water, and stirring the mixture at 80℃ for 30 minutes; (c) adding 10 mL chitosan solution dropwise to a precursor solution of step (b); (d) heating 15 mL leaf extract obtained from leaves of Lantana camaraand Rhamnus triquetrain a water bath at 45℃ for 30 minutes; (e) adding leaf extract to the solution of step (a) and magnetically stirring for 1 hour; (f) adding 1M solution of sodium hydroxide to the solution of step (e) and stirring for 2 hours, resulting in a color change of the mixture; (g) leaving obtained suspension undisturbed overnight in a dark environment to facilitate the formation of precipitate; (h) centrifuging the precipitate at 4000 revolutions/minute for 40 minutes to obtain pellet; (i) washing the pellet with distilled water to remove impurities and drying in oven at 60℃ for 12 hours; and (j) transferring dried pellet into a clean bottle for further storage.
IPC 分类号	A01N-059/16 ; A61K-036/72 ; B82Y-030/00 ; B82Y-040/00 ; C01G-009/02
国家	印度
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21111
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV SHOOLINI BIOTECHNOLOGY & MANAGEMENT (USLN-C)
推荐引用方式 GB/T 7714	MANN N A,SHARMA R,THAKUR N,et al. Preparing chitosan-coated metallic nanoparticle exhibiting antifungal activity, comprises preparing precursor solution by dissolving zinc nitrate in water, adding chitosan solution to precursor solution, adding leaf extract of Lantana camara and Rhamnus triquetra to solution, adding sodium hydroxide. IN202311050383-A[P]. 2023.

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