Preparing chitosan-coated metallic nanoparticles exhibiting antibacterial effect, comprises dissolving copper sulfate in water, adding chitosan solution, heating leaf extract obtained from leaves of Lantana camara, adding leaf extract to above solution, adding sodium hydroxide, and centrifuging
	MANN N A; SHARMA R; THAKUR N; ALTABBAA S
	2023-07-26
专利权人	UNIV SHOOLINI BIOTECHNOLOGY & MANAGEMENT (USLN-C)
申请日期	2023-07-26
专利号	IN202311050380-A
成果简介	NOVELTY - Preparing chitosan-coated metallic nanoparticles comprises: (a) preparing chitosan solution by dissolving 700 mg chitosan in 1% glacial acetic acid; (b) preparing metal precursor solution by dissolving 0.05 M copper sulfate in 100 mL distilled water and stirring the mixture at 80℃ for 30 minutes; (c) adding 10 mL chitosan solution to a precursor solution of step (b); (d) heating 15 mL leaf extract obtained from leaves of Lantana camara in a water bath; (e) adding leaf extract to the solution of step (a) and magnetically stirring; (f) adding 1M solution of sodium hydroxide to the solution of step (e), stirring for 2 hours, resulting in a color change of the mixture; (g) leaving the suspension from step (f) undisturbed overnight in a dark environment; (h) centrifuging the suspension to obtain pellet; (i) washing the pellet with distilled water and drying in oven for 12 hours at 60℃; and (j) transferring the dried pellet into a clean bottle for further storage. USE - Method for preparing chitosan-coated metallic nanoparticles exhibiting antibacterial and antifungal effect against various pathogens such as Escherichia coli, Staphylococcus aureus, Candida albicans, and Rosellinia necatrix. Test details are described but no results given. ADVANTAGE - The method provides chitosan-coated metallic nanoparticles with excellent stability and homogeneous arrangement, improved biocompatibility, and high purity, in scalable, environmentally-friendly and cost-effective manner. DETAILED DESCRIPTION - Preparing chitosan-coated metallic nanoparticles comprises: (a) preparing chitosan solution by dissolving 700 mg chitosan in 1% glacial acetic acid; (b) preparing metal precursor solution by dissolving 0.05 M copper sulfate in 100 mL distilled water and stirring the mixture at 80℃ for 30 minutes; (c) adding 10 mL chitosan solution dropwise to a precursor solution of step (b); (d) heating 15 mL leaf extract obtained from leaves of Lantana camara in a water bath at 45℃ for 30 minutes; (e) adding leaf extract to the solution of step (a) and magnetically stirring for 1 hour; (f) adding 1M solution of sodium hydroxide to the solution of step (e) followed by stirring for 2 hours, resulting in a color change of the mixture; (g) leaving the suspension from step (f) undisturbed overnight in a dark environment to facilitate the formation of precipitate; (h) centrifuging the suspension at 4000 revolutions/minute for 40 minutes to obtain pellet; (i) washing the pellet with distilled water to remove impurities and drying in oven for 12 hours at 60℃; and (j) transferring the dried pellet into a clean bottle for further storage.
IPC 分类号	A61K-036/72 ; A61K-036/85 ; A61P-031/04 ; B01J-037/00 ; B82Y-040/00
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/21108
专题	中国科学院新疆生态与地理研究所
作者单位	UNIV SHOOLINI BIOTECHNOLOGY & MANAGEMENT (USLN-C)
推荐引用方式 GB/T 7714	MANN N A,SHARMA R,THAKUR N,et al. Preparing chitosan-coated metallic nanoparticles exhibiting antibacterial effect, comprises dissolving copper sulfate in water, adding chitosan solution, heating leaf extract obtained from leaves of Lantana camara, adding leaf extract to above solution, adding sodium hydroxide, and centrifuging. IN202311050380-A[P]. 2023.

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