Detecting Mycobacterium tuberculosis genotype Beijing 14717-15-cluster, by detecting presence of single nucleotide substitution in Rv2161c gene by real-time PCR, and detecting Mycobacterium tuberculosis genotype Beijing 14717-15-cluster based on PCR result
	VYAZOVAYA A A; MUDARISOVA R S; BADLEEVA M V; GERASIMOVA A A; PASECHNIK O A; KOSTIUKOVA I V; SOLOVYEVA N S; ZHURAVLYOV V YU; OGARKOV O B; MOKROUSOV I V
	2023-08-03
专利权人	ST PETERSBURG EMERGENCY SCI RES INST (SPET-Non-standard) ; UNIV URAL FEDERAL (UYUR-C) ; HEALTH PROBLEMS & HUMAN REPROD FAMILY (HEAL-Non-standard)
申请日期	2023-08-03
专利号	RU2023120491-A
成果简介	NOVELTY - Method for detecting Mycobacterium tuberculosisgenotype Beijing 14717-15-cluster by real-time PCR involves (a) detecting presence of a single nucleotide substitution A-G in Rv2161c gene at genomic position 2423040 by real-time PCR, and (b) performing an assessment of the PCR result in 20-35 cycles to confirm that the sample belongs to the M.tuberculosisgenotype Beijing 14717-15-cluster. USE - The method is useful for detecting M.tuberculosisgenotype Beijing 14717-15-cluster by real-time PCR. DETAILED DESCRIPTION - Method for detecting Mycobacterium tuberculosisgenotype Beijing 14717-15-cluster by real-time PCR involves (a) detecting presence of a single nucleotide substitution A-G in Rv2161c gene at genomic position 2423040 by real-time PCR, by using (i) primers 2423040-forward (F) primer having base pair sequence: 5'-cggcgtgctcctgctgat (SEQ ID NO: Not defined) and 2423040-reverse (R) primer having base pair sequence: 5'-agttcgtcaccgacctgac (SEQ ID NO: Not defined), and (ii) fluorescently labeled probe having base pair sequence: 5'-[FAM(RTM: 5-Carboxyfluorescein)] cgtgcgtcttctccggc[A-LNA]CAT[Black Hole Quencher-1(RTM: Not defined)] for wild allele 2423040A and 5'-[HEX(RTM: 2',4',5',7',1,4-Hexachlorofluorescein)] cgtgcgtcttctccggc[G-LNA]CAT[Black Hole Quencher-1(RTM: Not defined)] for mutant allele 2423040G, and (b) performing an assessment of the PCR result in 20-35 cycles by recording the exponential growth of the fluorescence signal in the HEX(RTM: 2',4',5',7',1,4-Hexachlorofluorescein) channel with a wavelength of 555 nm and the absence of a fluorescence signal in the FAM(RTM: 5-Carboxyfluorescein) channel with a wavelength of 510 nm to confirm that the sample belongs to the M.tuberculosisgenotype Beijing 14717-15-cluster.
IPC 分类号	C12Q-001/686
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20974
专题	中国科学院新疆生态与地理研究所
作者单位	1.ST PETERSBURG EMERGENCY SCI RES INST (SPET-Non-standard) 2.UNIV URAL FEDERAL (UYUR-C) 3.HEALTH PROBLEMS & HUMAN REPROD FAMILY (HEAL-Non-standard)
推荐引用方式 GB/T 7714	VYAZOVAYA A A,MUDARISOVA R S,BADLEEVA M V,et al. Detecting Mycobacterium tuberculosis genotype Beijing 14717-15-cluster, by detecting presence of single nucleotide substitution in Rv2161c gene by real-time PCR, and detecting Mycobacterium tuberculosis genotype Beijing 14717-15-cluster based on PCR result. RU2023120491-A[P]. 2023.

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