| AGENT FOR IMMUNOPROPHYLAXIS OF TISSUE HELMINTHIASES OF FARM ANIMALS AND HUMANS, METHOD FOR OBTAINING AND USE THEREOF | |
| 2023-08-09 | |
| 专利权人 | FEDERAL STATE BUDGET SCI INST INST ENGIN (BUDG-Non-standard) |
| 申请日期 | 2023-08-09 |
| 专利号 | RU2830894-C1 |
| 成果简介 | NOVELTY - Disclosed is a vaccine for immunoprophylaxis of tissue forms of echinococcosis of farm animals and humans, containing recombinant oncosphere protective Echinococcus antigen EG95tr, encoded by nucleotide sequence SEQ ID NO: 1 or SEQ ID NO: 3, and auxiliary components. Disclosed is a method of producing a vaccine for immunoprophylaxis of tissue forms of echinococcosis of farm animals and humans. Method provides for obtaining DNA coding recombinant Echinococcus EG95 antigen, having nucleotide sequence SEQ ID NO: 1 or SEQ ID NO: 3, cloning said DNA into a shuttle vector and delivering the genetic construct into Pichia pastoris yeast cells to obtain a genetically engineered microorganism Pichia pastoris carrying the EG95 protective antigen gene, fermentation on YPD, MMY, MGY, BMM, BMG, BMGY media, definitive media, purification of the obtained recombinant Echinococcus antigen EG95tr from the culture medium by centrifugation or decantation of the fermentation medium, followed by dilution of the culture medium, followed by ultrafiltration through filters with a pore size of 50 kDa and subsequent ultrafiltration through filters with a pore size of 3–5 kDa, with subsequent purification of the desired recombinant antigen from the culture medium or filtrate, preservation of the purified recombinant antigen by instantaneous freezing in liquid nitrogen or lyophilic drying, addition of auxiliary components represented by adjuvants, excipients, isotonic agents, stabilizers and/or preservatives. Disclosed is use of vaccine for immunoprophylaxis of tissue forms of echinococcosis of farm animals and humans in therapeutic doses based on protective antigen for subcutaneous 40 mcg/kg and 100 mcg/kg for intranasal immunization, in combination with an immunostimulating carrier, at least twice with interval of 10–14 days for immunoprophylaxis of tissue forms of echinococcosis of farm animals and humans. USE - Veterinary science; medicine; biotechnology. ADVANTAGE - Oncosphere antigen EG95tr has high protective activity, which provides stable production of antibodies to the recombinant antigen in the blood serum of immunized animals for at least 4 months, as well as reduction of infection of target animals with tissue helminths by not less than 78% for immunoprophylaxis of cystic hydatidosis. 3 cl, 13 dwg, 7 ex |
| IPC 分类号 | A61K-039/00 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/20898 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGET SCI INST INST ENGIN (BUDG-Non-standard) |
| 推荐引用方式 GB/T 7714 | KARAGYAUR M N,ABRAMOV V E,USPENSKY A V,et al. AGENT FOR IMMUNOPROPHYLAXIS OF TISSUE HELMINTHIASES OF FARM ANIMALS AND HUMANS, METHOD FOR OBTAINING AND USE THEREOF. RU2830894-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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