New fusion transcripts UBE3C-LRP5 (v1 and v2) comprising an ubiquitin-protein ligase E3C gene and and low-density lipoprotein-related protein gene, used in fluorescence-based one-step quantitative real-time polymerase chain reaction kit for identification of head and neck squamous cell carcinoma
	RUGMINI S V N; UPADHYAY P K; DHARAVATH B; DUTT A
	2023-08-14
专利权人	ADVANCED CENT TREATMENT RES & EDUCATION (ADTR-Non-standard)
申请日期	2023-08-14
专利号	IN202321054451-A
成果简介	NOVELTY - Fusion transcripts UBE3C-LRP5 (v1 and v2) is new, where fusion transcripts UBE3C-LRP5 comprises an ubiquitin-protein ligase E3C gene (UBE3C) and the low-density lipoprotein-related protein gene (LRP5). USE - Fusion transcripts UBE3C-LRP5 (v1 and v2) used in fluorescence-based one-step quantitative real-time polymerase chain reaction (PCR) kit for activating an oncogenic WNT signalling cascade, complementary DNA synthesis, and identification of head and neck squamous cell carcinoma. ADVANTAGE - The kit is employed for cDNA synthesis, and quantitative real-time PCR is capable of performing the whole reaction in a single tube with a single step without compromising the specificity and sensitivity of the reaction. The kit also shows simple and convenient operation, and short detection period, and quickly detect disease genes related to head and neck cancer. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for fluorescence-based one-step quantitative real-time polymerase chain reaction (PCR) kit, which comprises upstream forward primer, downstream reverse primer and a 6-FAM (RTM: Fluorescent dye)-BHQ (RTM: Black Hole Quencher)1 labeled fluorescent probe for identification of UBE3C-LRP5 (v1) fusion variant, where the primer sequences are AD3187, AD3189, and AD3193; upstream forward primer, downstream reverse primer and a Cy (RTM: red-fluorescent dye 5)-labeled fluorescent probe for detection of UBE3C-LRP5 (v2) fusion variant, where the primer sequences are AD3187, AD3190, and AD3194; upstream forward primer, downstream reverse primer, and a TexRed-labeled fluorescent probe for detection of RNase P reference control gene, where the primer sequences are shown as AD2234, AD2245, and AD3195.
IPC 分类号	A61K-039/015 ; C07K-014/50 ; C07K-014/72 ; C12N-009/12 ; C12Q-001/6886
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20812
专题	中国科学院新疆生态与地理研究所
作者单位	ADVANCED CENT TREATMENT RES & EDUCATION (ADTR-Non-standard)
推荐引用方式 GB/T 7714	RUGMINI S V N,UPADHYAY P K,DHARAVATH B,et al. New fusion transcripts UBE3C-LRP5 (v1 and v2) comprising an ubiquitin-protein ligase E3C gene and and low-density lipoprotein-related protein gene, used in fluorescence-based one-step quantitative real-time polymerase chain reaction kit for identification of head and neck squamous cell carcinoma. IN202321054451-A[P]. 2023.

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