Rapid and sensitive method for detecting Entamoeba histolytica in human liver pus cells, comprises extracting DNA from liver pus cell; performing loop-mediated isothermal amplification reaction with reaction mix comprising mixture of primers, sample DNA, and detecting Entamoeba histolytica DNA
	SHARMA H; HIRAY A R; MUNJAL K; GARG V; SEHGAL R; SANDHYA
	2023-08-14
专利权人	SEHGAL R (SEHG-Individual) ; SANDHYA (SAND-Individual) ; RANA S (RANA-Individual) ; KUMAR H (KUMA-Individual) ; GARG V (GARG-Individual)
申请日期	2023-08-14
专利号	IN202311054689-A
成果简介	NOVELTY - Rapid and sensitive method for detecting Entamoeba histolyticain human liver pus cells comprises: (i) extracting DNA from liver pus cell; (ii) performing loop-mediated isothermal amplification (LAMP) reaction with 25 µl reaction mix comprising 1.5µl of the mixture of 6 primers, 15 µl isothermal reaction mix, 1 µl sample DNA and reaction volume made with distilled water; and (iii) detecting Entamoeba histolyticaDNA. The primer/polynucleotide set comprises SEQ ID No. 1, SEQ ID No.2, sequence comprising 42 base pairs (SEQ ID NO: 3, 4), given in the specification, SEQ ID No.5 and SEQ ID No. 6. USE - Rapid and sensitive method for detecting Entamoeba histolyticain human liver pus cells. ADVANTAGE - The method is rapid, cost-effective and sensitive method for detecting amoebic liver abscess; provides 99% accuracy in detection of amoebic liver abscess in large number of samples simultaneously; provides accuracy in the results in lesser quantity of the primers and chemicals. DETAILED DESCRIPTION - Rapid and sensitive method for detecting Entamoeba histolyticain human liver pus cells comprises: (i) extracting DNA from liver pus cell; (ii) performing loop-mediated isothermal amplification (LAMP) reaction with 25 µl reaction mix comprising 1.5µl of the mixture of 6 primers, 15 µl isothermal reaction mix, 1 µl sample DNA and reaction volume made with distilled water; and (iii) detecting Entamoeba histolyticaDNA. The primer/polynucleotide set comprises SEQ ID No. 1 (catgggacaatgctgagg), SEQ ID No.2 (aatccttggttgacatcgtt), sequence comprising 42 base pairs (SEQ ID NO: 3, 4), given in the specification, SEQ ID No.5 (ggattttcacctctcccgtt) and SEQ ID No. 6 (agaacgaaagttaggggatcg). INDEPENDENT CLAIMS are included for: 1. a primer mixture comprising: forward inner primer (FIP, SEQ ID No.3) and backward inner primer (BIP, SEQ ID No.4) at a concentration of 40 pmol to 10 µl; loop Forward primer (SEQ ID No. 5) and Loop reverse primer (SEQ ID No. 6) at a concentration of 20 pmol - 5 µl; forward outer primer (F3, SEQ ID No.1) and backward outer primer (B3, SEQ ID No.2) each at concentration of 10 pmol to 2.5 µl; and 15 µl distilled water; and 2. a kit comprising the primer set; a negative control-a sample not containing nucleic acids; and positive control-nucleic acids extracted from detection target.
IPC 分类号	A61K-047/02 ; A61P-001/16 ; A61P-027/02 ; A61P-031/04 ; A61P-033/04
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20810
专题	中国科学院新疆生态与地理研究所
作者单位	1.SEHGAL R (SEHG-Individual) 2.SANDHYA (SAND-Individual) 3.RANA S (RANA-Individual) 4.KUMAR H (KUMA-Individual) 5.GARG V (GARG-Individual)
推荐引用方式 GB/T 7714	SHARMA H,HIRAY A R,MUNJAL K,et al. Rapid and sensitive method for detecting Entamoeba histolytica in human liver pus cells, comprises extracting DNA from liver pus cell; performing loop-mediated isothermal amplification reaction with reaction mix comprising mixture of primers, sample DNA, and detecting Entamoeba histolytica DNA. IN202311054689-A[P]. 2023.

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