Anti-cancer and immunomodulatory composition prepared from root biomass of Simarouba glauca for inducing apoptosis in human cancer cells, comprises saponin, carbohydrate, alkaloids, protein, and metal ions selected from iron, copper, and zinc and/or a combination, sodium, and potassium
	SINGH A; SEN S; BARKUME M; PRADHAN T; KASINATHAN N K; PRASAD K; GOSAI A; KHOLIYA F; KODE J; MEENA R
	2023-08-25
专利权人	ADVANCED CENT TREATMENT RES & EDUCATION (ADTR-Non-standard) ; COUNCIL SCI & IND RES INDIA (COUI-C)
申请日期	2023-08-25
专利号	IN202311057434-A
成果简介	NOVELTY - Anti-cancer and immunomodulatory composition prepared from root biomass of Simarouba glauca, comprises 9-15 wt.% saponin, 7.5-11 wt.% carbohydrate, 6-8 wt.% alkaloids, 1.5-5.5 wt.% protein, and metal ions selected from iron, copper, and zinc and/or a combination, sodium, and potassium. USE - Anti-cancer and immunomodulatory composition prepared from root biomass of Simarouba glauca forinducing apoptosis human cancer cells. ADVANTAGE - The anti-cancer and immunomodulatory composition increases cytotoxic ability of immune cell towards cancer targets, exhibits significant in vivo anticancer activity against the four human cancer xenograft mice models. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: 1. a method for preparing an anti-cancer and immunomodulatory composition from root biomass of Simarouba glauca, comprising: (a) powdering the dried waste root biomass of Simarouba glaucausing mechanical grinder to obtain a root biomass powder having size of 0.9-10 mm; (b) adding aqueous methanolic solvent in volume ratio of 20: 80 Water: methanol in a weight ratio of 1:5-1:15 to the powder obtained in step (a) followed by filtration to separate the filtrate as crude aqueous methanolic extract and residual root biomass; (c) evaporating the crude aqueous methanolic extract on rotatory evaporator to separate the methanol; and (d) lyophilizing methanol free crude aqueous methanolic extract to obtain the solid crude aqueous methanolic extract, where the methanolic extract comprises 9-15 wt.% saponin, 7.5-11 wt.% carbohydrate, 6-8 wt.% alkaloids, 1.5-5.5 wt.% protein, and metal ions selected from 10 ppm iron, 5 ppm copper, and 2 ppm zinc, 20 ppm sodium, and 10 ppm potassium; and 2. an integrated method for treating root biomass of Simarouba glauca, comprising: (a1) powdering the dried waste root biomass of Simarouba glaucausing mechanical grinder to obtain a root biomass powder having size of 0.9-10 mm; (b1) adding aqueous methanolic solvent in volume ratio of 20: 80 water: methanol in a weight ratio of 1:5-1:15 to the powder followed by filtration to separate the filtrate as crude aqueous methanolic extract and residual root biomass; (c1) evaporating the crude aqueous methanolic extract on rotatory evaporator to separate the methanol; (d1) lyophilizing methanol free crude aqueous methanolic extract to obtain the solid crude aqueous methanolic extract; (e1) bleaching the residual root biomass by soaking the residual root biomass in acetate buffer of pH 4.5-5.0 containing 2.0-4.5 wt.% sodium chlorite (NaClO2) for bleaching at 50-70℃ for 2-4 hours; (f1) neutralizing the bleached biomass with water and treating with 0.5-1.5 M sodium hydroxide (NaOH) solution at 50-70℃ for 11-15 hours followed by washing with water till neutrality; (g1) suspending the washed biomass in 3-7% hydrocholoric acid (HCl) solution for 10-14 hours and washing the slurry with water till neutrality followed by lyophilization to get cellulose; and (h1) hydrolyzing the cellulose in 20-50% sulfuric acid for 30-90 minutes followed and lyophilizing after removing excess acid to obtain nanocellulose.
IPC 分类号	A61K-036/185 ; A61K-036/42 ; A61K-008/9789 ; A61P-031/14 ; A61P-035/00
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20679
专题	中国科学院新疆生态与地理研究所
作者单位	1.ADVANCED CENT TREATMENT RES & EDUCATION (ADTR-Non-standard) 2.COUNCIL SCI & IND RES INDIA (COUI-C)
推荐引用方式 GB/T 7714	SINGH A,SEN S,BARKUME M,et al. Anti-cancer and immunomodulatory composition prepared from root biomass of Simarouba glauca for inducing apoptosis in human cancer cells, comprises saponin, carbohydrate, alkaloids, protein, and metal ions selected from iron, copper, and zinc and/or a combination, sodium, and potassium. IN202311057434-A[P]. 2023.

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