METHOD FOR GENOTYPING OF VACCINE STRAINS OF FOOT-AND-MOUTH DISEASE VIRUS SEROTYPE SAT-2 BY METHOD OF STUDY OF PEAKS OF MELTING POINTS OF AMPLICONS USING FLUORESCENT DIMERIC ACRIDINE EVA488
2023-08-28
专利权人FEDERAL STATE BUDGETARY INST (FSBI-C)
申请日期2023-08-28
专利号RU2823753-C1
成果简介NOVELTY - Described is a method for molecular diagnostics, namely genotyping of vaccine strains of foot-and-mouth disease virus serotype SAT-2 by the method of studying peaks of melting points of amplicons using fluorescent dimeric acridine Eva488. For the first time, differentiation of genome of vaccine strains of foot-and-mouth disease virus serotype SAT-2 using the developed method is presented. Developed method is characterized by analytical specificity equal to 100%. In 95% confidence interval, the diagnostic sensitivity for the given method is 99.34–100.00%, the diagnostic specificity is 98.07–100.0%. USE - Veterinary virology. ADVANTAGE - Main advantage of the proposed invention is the ability to carry out in a short period of time, not more than 2.5 hours, genotyping of foot-and-mouth disease virus of serotype SAT-2 by the developed method. 2 cl, 2 dwg, 6 tbl, 3 ex
IPC 分类号A61D-099/00 ; C12Q-001/68
国家俄罗斯
专业领域农业科学
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/20663
专题中国科学院新疆生态与地理研究所
作者单位
FEDERAL STATE BUDGETARY INST (FSBI-C)
推荐引用方式
GB/T 7714
DORONIN M I,MIKHALISHIN D V,MALYGIN M P,et al. METHOD FOR GENOTYPING OF VACCINE STRAINS OF FOOT-AND-MOUTH DISEASE VIRUS SEROTYPE SAT-2 BY METHOD OF STUDY OF PEAKS OF MELTING POINTS OF AMPLICONS USING FLUORESCENT DIMERIC ACRIDINE EVA488. RU2823753-C1[P]. 2023.
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