| METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF VACCINE STRAIN "PB-97" OF RABIES VIRUS IN RAW MATERIAL FOR VACCINE BASED ON ANALYSIS OF MELTING CURVES OF HIGH-RESOLUTION AMPLICONS USING THIRD GENERATION INTERCALATING DYE EVAGREEN | |
| 2023-08-28 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-08-28 |
| 专利号 | RU2830983-C1 |
| 成果简介 | NOVELTY - Invention relates to production of an attenuated cultural rabies vaccine. Disclosed is a method for indirect determination of titre of infectious activity of vaccine strain "PB-97" of rabies virus in raw material for vaccine based on analysis of melting curves of high-resolution amplicons using intercalating dye of the third generation EvaGreen. USE - Biotechnology. ADVANTAGE - Main advantages of the proposed invention are reduce time of analysis of virus-containing suspensions for determination of titre of infectious activity of strain "PB-97" of rabies virus in raw material for vaccine to 2,5 hours; reduce the cost of one study by 29%; increase analytical sensitivity to 0,2 lg CCID50/cm3 or 1,58 CCID50/cm3 due to development of original highly specific primers and application of intercalating dye of the third generation EvaGreen; increase the reliability of the analysis due to the establishment of the relationship between the titre of the infectious activity of the vaccine strain "PB-97" of the rabies virus in the raw material for the vaccine and the quantification cycle—EvaGreen, presented in the form of a logarithmic function with high accuracy of approximation 0,9977 and amplification efficiency of 99,77%. 1 cl, 2 dwg, 6 tbl, 5 ex |
| IPC 分类号 | C12N-007/00 ; C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/20662 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | DORONIN M I,MIKHALISHIN D V,MALYGIN M P,et al. METHOD FOR INDIRECT DETERMINATION OF TITRE OF INFECTIOUS ACTIVITY OF VACCINE STRAIN "PB-97" OF RABIES VIRUS IN RAW MATERIAL FOR VACCINE BASED ON ANALYSIS OF MELTING CURVES OF HIGH-RESOLUTION AMPLICONS USING THIRD GENERATION INTERCALATING DYE EVAGREEN. RU2830983-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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