| METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "PB-97" FROM FIELD RABIES VIRUS ISOLATES BY DATA OF MAXIMUM GRAPH OF DERIVATIVE OF SIGMOID FUNCTION DURING AMPLIFICATION OF SECTION OF N-GENE OF VIRAL CDNA USING SYTO 16 DYE | |
| 2023-08-29 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-08-29 |
| 专利号 | RU2823779-C1 |
| 成果简介 | NOVELTY - Described is a method of differentiating the vaccine strain "PB-97" from field isolates of rabies virus according to the maximum of the graph of the derivative of the sigmoid function when amplifying a portion of the N-gene of the viral cDNA using SYTO 16 dye. Method is characterized by analytical specificity equal to 100%, diagnostic sensitivity in 95% confidence interval equal to 99.19–100.00%, diagnostic specificity—98.44–100.0%, overall accuracy—99.47–100.00%. USE - Veterinary virology. ADVANTAGE - Main advantage of the present invention is the possibility of differentiating the genome of the vaccine strain “PB-97” from field isolates of the rabies virus in a short period of time of 2.5 hours using the developed method. 2 cl, 3 dwg, 5 tbl, 3 ex |
| IPC 分类号 | C12N-015/00 ; G01N-033/569 |
| 国家 | 俄罗斯 |
| 专业领域 | 农业科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/20643 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | DORONIN M I,MALYGIN M P,MIKHALISHIN D V,et al. METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "PB-97" FROM FIELD RABIES VIRUS ISOLATES BY DATA OF MAXIMUM GRAPH OF DERIVATIVE OF SIGMOID FUNCTION DURING AMPLIFICATION OF SECTION OF N-GENE OF VIRAL CDNA USING SYTO 16 DYE. RU2823779-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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