Preparing polysaccharide for use in composition, comprises homogenizing seaweed Ulva fasciata, subjecting homogenized powder to depigmentation, extracting wet solids with water, treating concentrate with halogenated organic acid, mixing deproteinated extract of Ulva fasciata with ice-cold alcohol
	GOPALAKRISHNAN A; JAMES M T; PAI A A; DHARA S; PAI S K; CHAKRABORTY K
	2023-09-02
专利权人	INDIAN COUNCIL AGRIC RES (ICOU-C)
申请日期	2023-09-02
专利号	IN202311058947-A
成果简介	NOVELTY - Preparing polysaccharide from seaweed Ulva fasciata comprises: (i) washing seaweed Ulva fasciata in water followed by rinsing, drying and homogenizing to obtain a homogenized Ulva fasciata powder; (ii) subjecting the homogenized Ulva fasciata powder to depigmentation by treating with a fluid medium to obtain depigmented wet solids; (iii) extracting the wet solids with water to obtain an aqueous extract, and concentrating the aqueous extract to obtain aqueous concentrate of Ulva fasciata; (iv) treating the aqueous concentrate of Ulva fasciata with tri-substituted halogenated organic acid to obtain a treated extract; (v) centrifuging the treated extract to obtain deproteinated extract of Ulva fasciata; (vi) mixing the deproteinated extract of Ulva fasciata with ice-cold alcohol, and centrifuging for 15-35 minutes to obtain a crude polysaccharide of Ulva fasciata; and (vii) fractionating the crude polysaccharide to obtain polysaccharide. USE - Method for preparing polysaccharide for use in composition (claimed), and having immunomodulatory, antiviral and anti-inflammatory activities. Test details are described but no results given. ADVANTAGE - The composition containing the polysaccharide has shelf life of 11-14 months, and the polysaccharide can be prepared in simple, safe, and effective manner. DETAILED DESCRIPTION - Preparing polysaccharide from seaweed Ulva fasciata comprises: (i) washing seaweed Ulva fasciata in water followed by rinsing, drying and homogenizing to obtain a homogenized Ulva fasciata powder; (ii) subjecting the homogenized Ulva fasciata powder to depigmentation by treating with a fluid medium in a first predetermined ratio at a first predetermined temperature for a first predetermined time to obtain depigmented wet solids; (iii) subjecting the wet solids to extraction with water at a second predetermined temperature for a second predetermined time to obtain an aqueous extract, and concentrating the aqueous extract to obtain aqueous concentrate of Ulva fasciata; (iv) treating the aqueous concentrate of Ulva fasciata with a predetermined amount of tri-substituted halogenated organic acid at a third predetermined temperature for a third predetermined time to obtain a treated extract; (v) centrifuging the treated extract at a first predetermined speed for 15-35 minutes to obtain deproteinated extract of Ulva fasciata; (vi) mixing the deproteinated extract of Ulva fasciata with a predetermined amount of ice-cold alcohol at a fourth predetermined temperature for a fourth predetermined time, and centrifuging at a second predetermined speed for 15-35 minutes to obtain a crude polysaccharide of Ulva fasciata; and (vii) fractionating the crude polysaccharide to obtain polysaccharide. An INDEPENDENT CLAIM is included for a composition, which comprises 90-98 mass% polysaccharide of Ulva fasciata, and 2-10 mass% antioxidants.
IPC 分类号	A23L-033/125 ; A61K-031/715 ; A61K-036/02 ; A61K-009/00 ; C08B-037/00
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20556
专题	中国科学院新疆生态与地理研究所
作者单位	INDIAN COUNCIL AGRIC RES (ICOU-C)
推荐引用方式 GB/T 7714	GOPALAKRISHNAN A,JAMES M T,PAI A A,et al. Preparing polysaccharide for use in composition, comprises homogenizing seaweed Ulva fasciata, subjecting homogenized powder to depigmentation, extracting wet solids with water, treating concentrate with halogenated organic acid, mixing deproteinated extract of Ulva fasciata with ice-cold alcohol. IN202311058947-A[P]. 2023.

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