INTEGRATIVE CASSETTE FOR GENE EXPRESSION AND ITS USE IN CONSTRUCTING TRANSFORMANT OF YEAST KOMAGATAELLA MONDAVIORUM
	GORDEEVA T L; SHURYGINA E G; BORSHCHEVSKAYA L N; SINEOKII S P
	2023-09-20
专利权人	KURCHATOVSK NAT RES CENT INST (KURC-Non-standard)
申请日期	2023-09-20
专利号	RU2836330-C1
成果简介	NOVELTY - Disclosed is an integrative expression cassette for the expression of the RmAmyA gene encoding a recombinant α-amylase from R. miehei, comprising a PAOM promoter, a nucleotide sequence of a signal peptide, RmAmyA gene coding recombinant α-amylase from R. miehei, transcription terminator, selective marker for selection of transformants in yeast cells, region of integration, which is a fragment of nucleotide sequence M1 from chromosome K. mondaviorum VKPM Y-4330. Also disclosed is use of said integrative expression cassette for producing a transformant of yeast species K. mondaviorum, producing recombinant α-amylase from R. miehei and containing the RmAmyA gene. USE - Biotechnology. ADVANTAGE - Invention enables to obtain recombinant protein producer strains based on methylotrophic yeast Komagataella mondaviorum. 2 cl, 2 dwg, 2 tbl, 5 ex
IPC 分类号	C12N-001/19 ; C12N-015/56 ; C12N-015/81 ; C12N-009/30
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20328
专题	中国科学院新疆生态与地理研究所
作者单位	KURCHATOVSK NAT RES CENT INST (KURC-Non-standard)
推荐引用方式 GB/T 7714	GORDEEVA T L,SHURYGINA E G,BORSHCHEVSKAYA L N,et al. INTEGRATIVE CASSETTE FOR GENE EXPRESSION AND ITS USE IN CONSTRUCTING TRANSFORMANT OF YEAST KOMAGATAELLA MONDAVIORUM. RU2836330-C1[P]. 2023.

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