| METHOD FOR DNA IDENTIFICATION AND GENETIC CERTIFICATION OF PERENNIAL AND ANNUAL RYEGRASS VARIETIES BASED ON SSR- AND SCoT-MARKING SYSTEMS | |
| 2023-09-25 | |
| 专利权人 | FEDERAL RES CENT FEED PRODN & AGROECOLOG (REFE-Non-standard) |
| 申请日期 | 2023-09-25 |
| 专利号 | RU2826148-C1 |
| 成果简介 | NOVELTY - Described is a method for DNA identification and genetic certification of perennial and annual ryegrass varieties based on SSR- and SCoT-marking systems. Method involves the use of multilocus SCoT markers, which mark DNA associated with coding regions of the genome, to assess genetic polymorphism, detecting PCR products by preliminary agarose gel electrophoresis and drawing up molecular-genetic formulas based on the results of the detected allelic diversity. DNA extraction is carried out using a modified SDS method, in which 5 M ammonium acetate is used as a protein coagulant, DNA extraction is carried out without steps of additional purification and washing of solutions with ethanol, variety-specific DNA spectra are obtained from germinated seeds taken in an amount of at least thirty of each variety using two marking systems: 7 microsatellite SSR-loci and 9 SCoT-markers, detecting DNA fragments unique for the sample of the analyzed collection, the sizes of which are verified by high-resolution capillary electrophoresis and/or selective sequencing, molecular-genetic formula of the variety, as well as morphobiological and economic characteristics of the variety are added to the passport. USE - Molecular biology; plant genetics. ADVANTAGE - Method enables reliable and reliable differentiation and identification of varieties included in the State Register of the Russian Federation, and can be used in selection, seed growing, in the system of variety testing and registration, in the work of control and testing laboratories, in solving legal issues on protection of intellectual property rights. 1 cl, 7 dwg, 4 tbl, 6 ex |
| IPC 分类号 | A01H-001/00 ; C12Q-001/68 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/20266 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL RES CENT FEED PRODN & AGROECOLOG (REFE-Non-standard) |
| 推荐引用方式 GB/T 7714 | KLIMENKO I A,MAVLIUTOV I M,SHAMUSTAKIMOVA A O,et al. METHOD FOR DNA IDENTIFICATION AND GENETIC CERTIFICATION OF PERENNIAL AND ANNUAL RYEGRASS VARIETIES BASED ON SSR- AND SCoT-MARKING SYSTEMS. RU2826148-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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