Managing municipal solid organic waste through microbial liquefaction using bioreactor system, comprises segregating municipal solid organic waste to remove non-biodegradable material, grinding municipal organic waste, subjecting shredded organic waste to microbial liquefaction, and sieving slurry
	GANESHWADE D N; KULKARNI D V; KANETKAR S D; POLKADE A; RANADE D R
	2023-09-26
专利权人	SMARTENVIRO SYSTEMS PVT LTD (SMAR-Non-standard)
申请日期	2023-09-26
专利号	IN202321064541-A
成果简介	NOVELTY - Managing municipal solid organic waste through microbial liquefaction comprises: (a) segregating municipal solid organic waste to remove non-biodegradable material and obtaining the municipal solid organic waste being completely biodegradable; (b) grinding municipal solid organic waste in presence of k-type media to obtain shredded solid organic waste; (c) concomitantly subjecting the shredded solid organic waste to microbial liquefaction by adding a microbial consortium along with water to facilitate hydrolysis, decomposition and liquefaction of the shredded solid organic waste at room temperature around 28-45℃ for 24 hrs under aerobic or microaerophilic conditions to obtain a slurry consisting of a mixture of liquified organic waste and undecomposed solids, where the microbial consortium consists of a microbial suspension of Bacillus subtilis, Bacillus megaterium, and Trichoderma viride; and (d) sieving the slurry by passing it through a mesh having diameter of 2-6 mm. USE - Method for managing municipal solid organic waste through microbial liquefaction using a bioreactor system (claimed). ADVANTAGE - The method facilitates reduced waste volume, ease of transportation, reduction in transportation costs, and lower greenhouse gas emissions, and has minimal water requirement, does not require any physical pre-treatment, or addition of any chemicals, gas, and ensures a bioreactor system, which is cost-effective, and time efficient, free from potent greenhouse gas emissions, and can be carried out in a small area, and facilitates further processing to produce biogas, fertilizer, and other valuable products. DETAILED DESCRIPTION - Managing municipal solid organic waste through microbial liquefaction, facilitating conversion of municipal solid organic waste into liquified organic waste capable of being used as a liquid fertilizer and being re-processed to obtain biogas, biohydrogen, compost and bio-stimulant from it, which is being carried out in an existing waste treatment system such as a bioreactor, comprises: (a) segregating municipal solid organic waste to remove non-biodegradable material and obtaining municipal solid organic waste being completely biodegradable; (b) grinding municipal solid organic waste in presence of k-type media to obtain shredded solid organic waste, where the k-type media being present in an amount ranging from 5-8% of total capacity of the waste treatment system, and municipal solid organic waste undergoes size reduction upon grinding; (c) concomitantly subjecting the shredded solid organic waste to microbial liquefaction by adding a microbial consortium along with water to facilitate hydrolysis, decomposition and liquefaction of the shredded solid organic waste at room temperature around 28-45℃ for 24 hrs under aerobic or microaerophilic conditions to obtain a slurry consisting of a mixture of liquified organic waste and undecomposed solids, where the microbial consortium consists of a microbial suspension of Bacillus subtilis, Bacillus megaterium, Lactobacillussp. and Trichoderma viridepresent in proportions of 0.5: 0.5: 1: 0.5 with a population density of microbial consortium in the range of 106-108colony forming units (CFU)/mL; and (d) sieving the slurry by passing it through a mesh having diameter of 2-6 mm, where the liquefied organic waste percolates and separates out from the undecomposed solids, and the municipal solid organic waste, microbial consortium and water is added in proportions of 10 kg: 1.2 l: 5 l, and facilitating efficient liquefaction within 24 hrs. INDEPENDENT CLAIMS are included for: 1. a novel microbial consortium for municipal solid organic waste management through microbial liquefaction comprising Bacillus subtilis, Bacillus megaterium, Lactobacillussp. and Trichoderma viride, where the Lactobacillus sp. is Lactobacillus casei; 2. a method for preparing novel microbial consortium comprising: (i) culturing Bacillus subtilisand Bacillus megateriumseparately in a nutrient broth containing yeast extract, peptone and sodium chloride and incubating at 37℃ until a microbial suspension with a population density in the range of 106-108CFU/mL is attained; (ii) culturing Lactobacillus sp. in MRS (DeMan, Rogosa, and Sharpe) broth and incubating at 37℃ until a microbial suspension with a population density in the range of 106-108CFU/mL is attained; (iii) culturing Trichoderma viridein carboxy methyl cellulose (CMC) broth and incubating at 28℃ until a microbial suspension with a population density in the range of 106-108CFU/mL is attained; and (iv) combining microbial suspension to obtain the novel microbial consortium having high cell viability; and 3. a bioreactor system (200) for municipal solid organic waste management through microbial liquefaction comprising: horizontal cylindrical vessel defining: (i) first chamber (220) being a liquefaction chamber configured to receive municipal solid organic waste for liquefaction by a microbial consortium, where the first chamber consists of a central hollow shaft having triangular arms configured across it, to facilitate grinding of the municipal solid organic waste upon rotational movement of the central hollow shaft pursuant to actuation by a motor operably coupled to it, such that the municipal solid organic waste in presence of k-type media added as an auxiliary grinder reduces in size and converts to shredded solid organic waste upon grinding, where the rotational movement of the central hollow shaft at around 10- 12 revolutions per minute (rpm) further enables intermittent mixing of the shredded solid organic waste with the microbial consortium added thereto to facilitate liquefaction thereof at room temperature within 24 hrs under aerobic conditions and obtains slurry consisting of liquefied organic waste and undecomposed solids; (ii) second chamber (240) being a collection chamber in communication with the first chamber and configured with a mesh between it, the mesh having a diameter in range of 2-6 mm facilitating separation of the liquefied organic waste from the undecomposed solids within the slurry such that the liquefied organic waste percolates through the mesh and gets collected within the second chamber, where the undecomposed solids remain behind within the first chamber to undergo further cycles of liquefaction facilitating its complete decomposition; and (iii) a slurry pump (260) being configured in communication with the second chamber to remove the liquefied organic waste collected within it, where the slurry pump includes an automatic control valve (265) facilitating diversion of the liquefied organic waste back to the first chamber via segment of the slurry pump in communication with it to allow intermittent recycling of the liquefied organic waste offsetting the microbial consortium addition during further liquefaction cycles for municipal solid organic waste management. DESCRIPTION OF DRAWING(S) - The drawing shows a perspective view of bioreactor system. 200Bioreactor system 220First chamber 240Second chamber 260Slurry pump 265Automatic control valve 280Liquid mixture tank 285Liquid mixture pump
IPC 分类号	B09B-001/00 ; B09B-003/00 ; C02F-003/34 ; C05F-017/00 ; C12N-001/20
国家	印度
专业领域	能源矿产
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20246
专题	中国科学院新疆生态与地理研究所
作者单位	SMARTENVIRO SYSTEMS PVT LTD (SMAR-Non-standard)
推荐引用方式 GB/T 7714	GANESHWADE D N,KULKARNI D V,KANETKAR S D,et al. Managing municipal solid organic waste through microbial liquefaction using bioreactor system, comprises segregating municipal solid organic waste to remove non-biodegradable material, grinding municipal organic waste, subjecting shredded organic waste to microbial liquefaction, and sieving slurry. IN202321064541-A[P]. 2023.

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