New recombinant proline-proline-glutamate protein comprising it amino acid sequence, used as a sub-unit vaccine for reducing Tuberculosis-associated adipose tissue dysfunction, insulin resistance and diabetic complications and ameliorates of TB-associated diabetes
	GHOSH S; BISHT M K; MUKHOPADHYAY S
	2023-10-04
专利权人	CENT DNA FINGERPRINTING & DIAGNOSTICS (DNAF-Non-standard)
申请日期	2023-10-04
专利号	IN202341066556-A
成果简介	NOVELTY - Recombinant proline-proline-glutamate (rPPE2) protein is new, where rPPE2 protein comprising 561 amino acid sequence of (SEQ ID NO: 2), given in the specification. USE - Recombinant proline-proline-glutamate (rPPE2) protein used as a subunit vaccine for reducing Tuberculosis (TB)-associated adipose tissue dysfunction, insulin resistance and diabetic complications and ameliorates of TB-associated diabetes (claimed). No biological data given. ADVANTAGE - The rPPE2 protein exhibits adipose tissue atrophy along with reduction of adipogenic gene expression, impaired glucose tolerance, and reduction of adipogenic gene expression, shows significant improvement in adipose tissue mass as well as tissue histology indicating the neutralization of a PPE2 protein by an antibody developed through the rPPE2 protein vaccination rescuing the effect of the PPE2 in induction of TB-associated adipose tissue dysfunction and impaired glucose tolerance. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for development of recombinant proline-proline-glutamate (rPPE2) protein, which involves: (1) culturing of a bacterial strain by generating Mycobacterium smegmatis expressing a PPE2 protein of Mycobacterium tuberculosis as a Msmeg-PPE2, and generating a CDC1551 strain complemented with a wild-type PPE2 as CDC1551:ΔDPPE2::PPE2 by electroporating a pVV16-PPE2 construct into CDC1551:ΔDPPE2; (2) infecting a mice with the bacterial strain by infecting mice with the CDC1551:ΔDPPE2::PPE2 and optionally with CDC1551:ΔDPPE2 via aerosol route to form impurified rPPE2 protein; and (3) purifying the impurified rPPE2 protein to form a rPPE2 protein.
IPC 分类号	A61K-039/00 ; A61K-039/12 ; A61K-009/00 ; E21B-043/24 ; G03F-007/40
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20138
专题	中国科学院新疆生态与地理研究所
作者单位	CENT DNA FINGERPRINTING & DIAGNOSTICS (DNAF-Non-standard)
推荐引用方式 GB/T 7714	GHOSH S,BISHT M K,MUKHOPADHYAY S. New recombinant proline-proline-glutamate protein comprising it amino acid sequence, used as a sub-unit vaccine for reducing Tuberculosis-associated adipose tissue dysfunction, insulin resistance and diabetic complications and ameliorates of TB-associated diabetes. IN202341066556-A[P]. 2023.

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