New cytocompatible and antibacterial anionic peptide comprising selective anionic dodecapeptide PEP-M with specified sequence for use in anionic hydrogel system for preparing hydrogel for accelerating healing of chronic diabetic wounds infected with methicillin-resistant Staphylococcus aureus
	CHAUDHURY S; BAIRAGI M K; GHOSH S; SEN S; PAREEK V; MUKHERJEE N
	2023-10-10
专利权人	INDIAN INST TECHNOLOGY JODHPUR (INTE-Non-standard) ; IHUB DRISHTI FOUND (IHUB-Non-standard)
申请日期	2023-10-10
专利号	IN202311068152-A
成果简介	NOVELTY - A cytocompatible and antibacterial anionic peptide comprising a selective anionic dodecapeptide PEP-M with specified sequence (SEQ ID NO: 1) is new, where the peptide has a net charge of -3 at physiological pH of 7.4 constituting a selective αβ-tubulin heterodimer interface region having self-assembly based hydrogel formability. USE - Cytocompatible and antibacterial anionic peptide for use in anionic hydrogel system for preparing hydrogel for accelerating healing of chronic diabetic wounds infected with methicillin-resistant Staphylococcus aureus. Test details are described but no results given. ADVANTAGE - The anionic hydrogel system ensures antibacterial and anti-biofilm effectivity, undergoes conformational changes and self-assemble upon variations in pH and/or temperature, and exhibits gelation properties under physiological settings, ensures direct encapsulation of cells in a three-dimensional thixotropic (injectable) matrix. DETAILED DESCRIPTION - A cytocompatible and antibacterial anionic peptide comprising a selective anionic dodecapeptide PEP-M with NH2-Trp-Tyr-Thr-Gly-Glu-Gly-Met-Asp-Glu-Met-Glu-Phe-CONH2(SEQ ID NO: 1) is new, where the peptide has a net charge of -3 at physiological pH of 7.4 constituting a selective αβ-tubulin heterodimer interface region having self-assembly based hydrogel formability. INDEPENDENT CLAIMS are included for: an anionic hydrogel system, which comprises gelled cytocompatible anionic dodecapeptide PEP-M; a method for producing an anionic dodecapeptide PEP-M involving solid-phase peptide synthesis (SPPS); and a method for synthesizing hydrogel, which comprises: (i) dissolving at least 1.8 mg PEP-M selectively in 1 mL filtered 1x phosphate buffer saline in a vial to obtain 1.8 wt. % solution or HPLC purified water; (ii) subjecting the resulting mixture to sonication at 20-40 kHz for 15 minutes to obtain a homogeneous mixture; (c) sonicating gently for 15 minutes at 37℃ temperature water bath or heating at 80℃ followed by cooling at room temperature for 30 minutes; (d) subjecting obtained mixture to rapid supramolecular self-assembly at minimum gelation concentration (MGC) og 1.8 wt.% at physiological pH (pH of 7.4) upon gentle sonication for 15 minutes at 37℃ water bath or heating at 80℃ followed by cooling at room temperature for 30 minutes to obtain hydrogel.
IPC 分类号	A61K-009/00 ; A61K-009/06 ; A61L-026/00 ; A61P-017/02 ; C07K-007/06
国家	印度
专业领域	医药卫生
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20087
专题	中国科学院新疆生态与地理研究所
作者单位	1.INDIAN INST TECHNOLOGY JODHPUR (INTE-Non-standard) 2.IHUB DRISHTI FOUND (IHUB-Non-standard)
推荐引用方式 GB/T 7714	CHAUDHURY S,BAIRAGI M K,GHOSH S,et al. New cytocompatible and antibacterial anionic peptide comprising selective anionic dodecapeptide PEP-M with specified sequence for use in anionic hydrogel system for preparing hydrogel for accelerating healing of chronic diabetic wounds infected with methicillin-resistant Staphylococcus aureus. IN202311068152-A[P]. 2023.

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