Synthesizing of polylactic acid from whey involves boiling milk and adding lemon juice in order to obtain coagulated milk, followed by separation of whey from the coagulated milk, filtering the whey using Whatman filter paper to remove impurities from the whey
	DEV M; SWARNAKAR A K; GEMEDAR N; THAPAR P
	2023-10-10
专利权人	THAPAR P (THAP-Individual)
申请日期	2023-10-10
专利号	IN202341068039-A
成果简介	NOVELTY - Synthesizing of polylactic acid from whey involves: (a) boiling milk and adding lemon juice in order to obtain coagulated milk, followed by separation of whey from the coagulated milk; (b) filtering the whey using Whatman filter paper to remove impurities from the whey and collecting the filtered whey in a flask, followed by sterilization of the flask in an autoclave at a temperature in the range of 115-125℃ and a pressure of 12-18 psi for a time duration in the range of 10-20 minutes in order to obtain sterilized whey; (c) inoculating the whey with culture of Bifidobacterium species under aseptic conditions in laminar air flow and keeping the flask in a temperature control orbital shaker (Orbitek) under anaerobic conditions at a temperature in the range of 25-40℃ for a time duration of 110-130 hours. USE - Method for synthesizing of polylactic acid from whey. ADVANTAGE - The method does not require expensive chemicals and complicated process, and it is capable of synthesizing the polylactic acid from the by-products released during the synthesis of cheese/ paneer for manufacturing the biofilm/laminates used in packaging of food. The method also use of plastic in order to reduce pollution and increases sustainability. DETAILED DESCRIPTION - Synthesizing of polylactic acid from whey involves: (a) boiling milk and adding lemon juice in order to obtain coagulated milk, followed by separation of whey from the coagulated milk; (b) filtering the whey using Whatman filter paper to remove impurities from the whey and collecting the filtered whey in a flask, followed by sterilization of the flask in an autoclave at a temperature in the range of 115-125℃ and a pressure of 12-18 psi for a time duration in the range of 10-20 minutes in order to obtain sterilized whey; (c) inoculating the whey with culture of Bifidobacterium species under aseptic conditions in laminar air flow and keeping the flask in a temperature control orbital shaker (Orbitek) under anaerobic conditions at a temperature in the range of 25-40℃ for a time duration of 110-130 hours, followed by centrifuging the flask at stirring speed of 4500-5500 revolution/minute (rpm) for a time duration in the range of 10-20 minutes in a centrifuge in order to obtain a supernatant; (d) filtering the supernatant using a Whatman filter paper to obtain lactic acid containing fermented whey, followed by adding enzyme protease, distilled water, and toluene and keeping in orbital shaker at a temperature of 35-45℃ at stirring speed of 600-700 rpm for a time duration of 90-100 hours to obtain a mixture; (e) adding chloroform to the obtained mixture and incubating at a temperature in the range of 55-65℃ for a time duration of 5-15 minutes, followed by adding distilled water and incubating at room temperature of 5-15 minutes in order to obtain a two-phase solution; and (f) subjecting the two-phase solution to rotary vapourization process in order to obtain the polylactic acid.
IPC 分类号	A23C-021/02 ; A23C-021/08 ; A23C-009/13 ; A61K-035/745 ; C12N-015/10
国家	印度
专业领域	化学化工
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/20085
专题	中国科学院新疆生态与地理研究所
作者单位	THAPAR P (THAP-Individual)
推荐引用方式 GB/T 7714	DEV M,SWARNAKAR A K,GEMEDAR N,et al. Synthesizing of polylactic acid from whey involves boiling milk and adding lemon juice in order to obtain coagulated milk, followed by separation of whey from the coagulated milk, filtering the whey using Whatman filter paper to remove impurities from the whey. IN202341068039-A[P]. 2023.

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