| METHOD FOR ASSESSING GENETIC STABILITY OF MICROCLONALLY PROPAGATED STRAWBERRY PLANTS | |
| 2023-10-17 | |
| 专利权人 | NORTH-CAUCASIAN HORTICULTURE FEDERAL SCI (NCAU-Non-standard) |
| 申请日期 | 2023-10-17 |
| 专利号 | RU2835821-C1 |
| 成果简介 | NOVELTY - Invention relates to nursery, to methods of assessing genetic stability of microclonally propagated strawberries. Disclosed is a method for assessing the genetic stability of microclonally propagated strawberry plants, involving sampling a fresh biomaterial, DNA extraction by CTAB and comparative PCR analysis, characterized by the fact that high-information ISSR-marker ASSR 20 or high-information ISSR-markers are used to assess genetic stability of regenerants and mother plant of garden strawberry: UBC 849 and ISSR 6. In this method, the following parameters are used during PCR analysis: 3 minutes of preliminary denaturation at temperature of 95 °C; next 35 cycles: denaturation 35 seconds at 95 °C, primer annealing: 1 minute at 50 °C, elongation: 1.5 minutes at 72 °C, and the final synthesis cycle at temperature of 72 °C for 5 minutes. Method is suitable for implementation in laboratories equipped with a minimum amount of equipment for molecular genetic research. USE - Plant growing. ADVANTAGE - Application of the method complies with the practice of controlling genetic stability of planting material of garden strawberry and thus promotes improvement of quality of nursery products. 2 cl, 3 dwg, 1 tbl, 3 ex |
| IPC 分类号 | A01H-001/00 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19510 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | NORTH-CAUCASIAN HORTICULTURE FEDERAL SCI (NCAU-Non-standard) |
| 推荐引用方式 GB/T 7714 | SUPRUN I I,YAKUBA YU F,STEPANOV I V,et al. METHOD FOR ASSESSING GENETIC STABILITY OF MICROCLONALLY PROPAGATED STRAWBERRY PLANTS. RU2835821-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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