| METHOD FOR INDIRECT DETERMINATION OF CONCENTRATION OF A-ANTIGEN OF INDUSTRIAL STRAIN "VGNKI" OF CAUSATIVE AGENT OF INFECTIOUS HEPATITIS OF DOGS IN RAW MATERIAL FOR CULTURAL VACCINES ACCORDING TO QUANTITATIVE ASSESSMENT CYCLE DURING AMPLIFICATION OF TARGET REGION OF ORF25-GENE | |
| 2023-10-18 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-10-18 |
| 专利号 | RU2823930-C1 |
| 成果简介 | NOVELTY - Described is a method for indirect determination of concentration of A-antigen of the industrial strain "VGNKI" of the causative agent of canine infectious hepatitis in raw material for cultural vaccines according to the cycle of quantitative assessment during amplification of the target region of the orf25-gene. USE - Biotechnology. ADVANTAGE - Main advantages of the proposed invention are reduction of time for analysis of industrial raw material for production of cultural vaccines against CAV-1 by determining the concentration of the A-antigen of the strain "VGNKI" CAV-1 virus up to 3 hours (16 times faster than the prototype); elimination of contamination probability; high analytical, diagnostic sensitivity and specificity of the analysis; elimination of subjectivity factor of obtained results due to use of instrumentation for detection of results; increased reliability of the analysis due to the establishment of the relationship between the concentration of the A-antigen of the strain "VGNKI" CAV-1 and the cycle of quantitative assessment of the amplicons of the target region of the orf25-gene, presented in form of quadratic function with high values of accuracy of approximation and efficiency of amplification reaction. 3 cl, 2 dwg, 5 tbl, 6 ex |
| IPC 分类号 | A61K-039/02 ; G01N-033/531 |
| 国家 | 俄罗斯 |
| 专业领域 | 农业科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19507 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | DORONIN M I,MALYGIN M P,GALKINA T S,et al. METHOD FOR INDIRECT DETERMINATION OF CONCENTRATION OF A-ANTIGEN OF INDUSTRIAL STRAIN "VGNKI" OF CAUSATIVE AGENT OF INFECTIOUS HEPATITIS OF DOGS IN RAW MATERIAL FOR CULTURAL VACCINES ACCORDING TO QUANTITATIVE ASSESSMENT CYCLE DURING AMPLIFICATION OF TARGET REGION OF ORF25-GENE. RU2823930-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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