METHOD OF DIFFERENTIATING VACCINE STRAIN "RICH" FROM OTHER STRAINS AND FIELD ISOLATES OF CAUSATIVE AGENT OF CORONAVIRUS INFECTION IN DOGS BY ANALYZING MAXIMUM EXTREMA OF AMPLICON MELTING TEMPERATURE CURVES USING SUPERNOVA v428 VIOLET LASER DYE
2023-10-30
专利权人FEDERAL STATE BUDGETARY INST (FSBI-C)
申请日期2023-10-30
专利号RU2826458-C1
成果简介NOVELTY - Invention relates to molecular diagnostics. Described is a method of differentiating the vaccine strain "RICH" from other strains and field isolates of the causative agent of coronavirus infection in dogs by analyzing maximum extrema of amplicon melting temperature curves using SuperNova v428 violet laser dye. USE - Veterinary virology. ADVANTAGE - Main advantages of the proposed invention are the ability to carry out in a short period of time, not more than 2,5 hours, differentiation of vaccine strain "RICH" from other strains and field isolates of causative agent of coronavirus infection of dogs; developed method is characterized by 100% analytical specificity; in 95% confidence interval diagnostic sensitivity for this method is 99,17–100%, diagnostic specificity—98,50–100%, accuracy—99,46–100%. 2 cl, 3 dwg, 5 tbl, 3 ex
IPC 分类号C12Q-001/68
国家俄罗斯
专业领域生物科学
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/19418
专题中国科学院新疆生态与地理研究所
作者单位
FEDERAL STATE BUDGETARY INST (FSBI-C)
推荐引用方式
GB/T 7714
DORONIN M I,GALKINA T S,MIKHALISHIN D V,et al. METHOD OF DIFFERENTIATING VACCINE STRAIN "RICH" FROM OTHER STRAINS AND FIELD ISOLATES OF CAUSATIVE AGENT OF CORONAVIRUS INFECTION IN DOGS BY ANALYZING MAXIMUM EXTREMA OF AMPLICON MELTING TEMPERATURE CURVES USING SUPERNOVA v428 VIOLET LASER DYE. RU2826458-C1[P]. 2023.
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