| METHOD FOR EDITING DNA OF AFRICAN SWINE FEVER VIRUS IN AREA OF GENES MGF 110_4L-6L USING PLASMIDS U6, pJET1.2 AND ENZYME Cas9 | |
| 2023-10-30 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-10-30 |
| 专利号 | RU2828403-C1 |
| 成果简介 | NOVELTY - Invention relates to a method for editing DNA of the African swine fever virus in MGF 110_4L-6L genes using plasmids U6, pJET1.2 and Cas9 enzyme. Said method provides for the creation of a plasmid-guide (gRNA) based on the plasmid U6, containing 20 bps, complementary to the portion of the genome of the ASF virus with genes MGF 110_4L-6L in positions 8724-10107 nucleotide base of the genome of the ASF virus, creation of shuttle plasmid Shuttle plasmid based on plasmid pJET1.2, which contains two fragments of ASF virus genome—Left-arm (L-arm) and Right-arm (R-arm), flanking MGF 110_4L-6L genes for their deletion, ASF virus genome editing in MGF 110_4L-6L genes region and the selected ASF virus strain in the primary porcine spleen cell culture, extraction of ASF virus DNA, sequencing and confirmation of MGF 110_4L-6L gene deletion. USE - Biotechnology. ADVANTAGE - Invention is effective for editing DNA of African swine fever virus in the area of MGF 110_4L-6L genes using plasmids U6, pJET 1.2 and Cas9 enzyme. 1 cl, 5 dwg, 4 tbl, 4 ex |
| IPC 分类号 | C12N-015/63 ; C12N-009/22 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19410 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | MAZLUM A,CHERNYSHEV R S,IGOLKIN A S,et al. METHOD FOR EDITING DNA OF AFRICAN SWINE FEVER VIRUS IN AREA OF GENES MGF 110_4L-6L USING PLASMIDS U6, pJET1.2 AND ENZYME Cas9. RU2828403-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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