| METHOD OF PRODUCING SUBSTRATE FOR MASSIVE PARALLEL SYNTHESIS OF OLIGONUCLEOTIDES | |
| 2023-11-01 | |
| 专利权人 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 申请日期 | 2023-11-01 |
| 专利号 | RU2826178-C1 |
| 成果简介 | NOVELTY - Invention relates to versions of a method of producing porous substrates for oligonucleotide synthesis from functionalised aluminum oxide. One of the versions of the method involves formation of a porous anodized oxide on one side of a thin sheet of aluminum in a potentiostatic mode at temperature of 0–10 °C. Further, unreacted aluminum and barrier layer are etched, aluminum oxide is thermally annealed for its conversion to α-phase and polymer bead is fixed. Obtained substrate is treated in 1–3% solution of 3–aminopropyltriethoxysilane in anhydrous toluene for 2–4 hours followed by washing with toluene and treatment in 5–15% solution of succinic anhydride in dimethylformamide for 2–4 hours, followed by washing with water. USE - Synthetic biology. ADVANTAGE - Obtaining substrates with a porous structure, a vertical arrangement of pores and a given interpore distance and the presence of functional groups on the surface of the pores, which provide covalent attachment of nucleotides during synthesis with targeted dosing of small amounts of reagents by such methods as jet printing, aerosol printing, capillary application. 4 cl, 1 ex |
| IPC 分类号 | B01J-019/00 ; C07H-001/00 ; C12N-015/10 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19385 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 推荐引用方式 GB/T 7714 | GADIROV R M,LOSHCHILOV A G,NOVIKOV V A,et al. METHOD OF PRODUCING SUBSTRATE FOR MASSIVE PARALLEL SYNTHESIS OF OLIGONUCLEOTIDES. RU2826178-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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