YEAST TRANSFORMANT YARROWIA LIPOLYTICA PRODUCING CANTHAXANTHIN
	TARATYNOVA M O; YUZBASHEVA E YU; GORCHAKOVA V A; TARASOV I M; FEDIAEVA I M; DEMENTYEV D A; FYODOROV A S; SINEOKII S P
	2023-11-01
专利权人	KURCHATOVSK NAT RES CENT INST (KURC-Non-standard)
申请日期	2023-11-01
专利号	RU2827562-C1
成果简介	NOVELTY - Disclosed is a yeast transformant Yarrowia lipolytica, which produces the carotenoid canthaxanthin and contains at least one copy of each of the following genes in the chromosome: M. circinelloides CarB gene coding phytoendehydrogenase, M. circinelloides CarRP gene coding bifunctional enzyme phytoene synthase/lycopene β-cyclase, Y. lipolytica HMGR1 gene coding 3-hydroxy-3-methylglutaryl-coenzyme A reductase enzyme, Y. lipolytica ERG20 gene coding farnesyl pyrophosphate synthase, Y. lipolytica ERG12 gene coding mevalonate kinase, nucleotide sequence CarRP-GGPPs7 coding polypeptide, obtained by combining bifunctional enzyme phytoene synthase/lycopene β-cyclase carRP M. circinelloides and geranylgeranyl pyrophosphate synthase GGPPs7 Synechococcus sp., nucleotide sequence CarRP-ERG20 (F88C) coding polypeptide, obtained by combining M. circinelloides carRP and a modified version of farnesyl pyrophosphate synthase ERG20F88C Y. Lipolytica, Haematococcus pluvialis codon-optimized HPBKT gene, which codes β-carotene ketolase, the nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: l, Schizosaccharomyces pombe codon-optimized gene SpHXKl, coding hexokinase, the nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: 2, codon-optimized gene YHT1 Y. lipolytica, coding hexose transporter, nucleotide sequence of which is given in sequence list under number SEQ ID NO: 3, codon-optimized gene YHT3 Y. lipolytica, coding hexose transporter, nucleotide sequence of which is given in sequence list under number SEQ ID NO: 5, codon-optimized gene YHT4 Y. lipolytica, coding hexose transporter, nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: 7. Also disclosed is a recombinant yeast strain Y. lipolytica VKPM Y-5144—a producer of canthaxanthin. USE - Biotechnology. ADVANTAGE - Invention widens the range of microorganisms producing the carotenoid canthaxanthin. 2 cl, 5 dwg, 5 tbl, 3 ex
IPC 分类号	C12N-001/19 ; C12N-015/81 ; C12P-023/00
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/19378
专题	中国科学院新疆生态与地理研究所
作者单位	KURCHATOVSK NAT RES CENT INST (KURC-Non-standard)
推荐引用方式 GB/T 7714	TARATYNOVA M O,YUZBASHEVA E YU,GORCHAKOVA V A,et al. YEAST TRANSFORMANT YARROWIA LIPOLYTICA PRODUCING CANTHAXANTHIN. RU2827562-C1[P]. 2023.

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