| METHOD OF DIFFERENTIATING GENOME OF FOOT-AND-MOUTH DISEASE VIRUS OF INDUSTRIAL STRAINS OF SEROTYPE O BY ANALYZING MAXIMUM POINT OF TM AMPLICONS USING GREEN INTERCALATING DYE YO-PRO-1 IODIDE | |
| 2023-11-02 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-11-02 |
| 专利号 | RU2832768-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, namely to a method for differentiating the genome of the foot-and-mouth disease virus of industrial strains of serotype O by analyzing the maximum point of Tm amplicons using the green intercalating dye YO-PRO-1 IODIDE. Method involves a polymerase chain reaction using specific oligonucleotide primers, a melting process of amplicons and determination of their melting point for differentiation of industrial strains of the serotype O foot-and-mouth disease virus. USE - Biotechnology. ADVANTAGE - Developed method makes it possible: to increase sensitivity and specificity due to the use of highly specific original primers, calculated for the target portion of 1D gene of the foot-and-mouth disease virus serotype O, increase the accuracy of the analysis due to the use of the green intercalating dye YO-PRO-1 IODIDE, as well as by analyzing the maximum melting point (Tm – melting temperature) of amplicons to differentiate industrial strains of the serotype O foot-and-mouth disease virus. 2 cl, 3 dwg, 4 tbl, 3 ex |
| IPC 分类号 | C12Q-001/68 ; G01N-033/48 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19367 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | DORONIN M I,MIKHALISHIN D V,MUDRAK N S,et al. METHOD OF DIFFERENTIATING GENOME OF FOOT-AND-MOUTH DISEASE VIRUS OF INDUSTRIAL STRAINS OF SEROTYPE O BY ANALYZING MAXIMUM POINT OF TM AMPLICONS USING GREEN INTERCALATING DYE YO-PRO-1 IODIDE. RU2832768-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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