| METHOD OF INFECTIOUS BOVINE LUMPY SKIN DISEASE VIRUS GENOME EDITING USING OVERLAP-PCR AND CRISPR-CAS9 TECHNOLOGY | |
| 2023-11-03 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-11-03 |
| 专利号 | RU2825451-C1 |
| 成果简介 | NOVELTY - Invention relates to a method for editing the genome of infectious bovine lumpy skin disease virus. Said method is carried out using Overlap-PCR and CRISPR-Cas9 technology, wherein the method involves: design of a primer sequence for the synthesis of a plasmid coding an RNA guide: cloning the sequences into the P1 plasmid; shuttle vector construction; transformation of MDBK cell culture with plasmid P1, coding RNA guide, shuttle vector and p-Cas9/No NLS; infection of MDBK cell culture with LSD virus; obtaining genetically modified infectious bovine lumpy skin disease virus. USE - Biotechnology. ADVANTAGE - Invention provides editing the genome of infectious bovine lumpy skin disease virus in vivo with high accuracy. 2 cl, 12 dwg, 8 tbl, 5 ex |
| IPC 分类号 | C12N-015/00 ; C12N-007/01 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19348 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | SPRYGIN A V,MAZLUM A,BIADOVSKAIA O P,et al. METHOD OF INFECTIOUS BOVINE LUMPY SKIN DISEASE VIRUS GENOME EDITING USING OVERLAP-PCR AND CRISPR-CAS9 TECHNOLOGY. RU2825451-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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