METHOD OF PRODUCING PANEL OF DNA PROBES FOR DETERMINING CHROMOSOMAL TRANSLOCATIONS, DELETIONS AND AMPLIFICATIONS
	VASILYEV S A; ZUEV A S; SAZHENOVA E A; TOLMACHEVA E N
	2023-11-17
专利权人	AS RUSSIA TOMSK NAT RES MEDICAL CENT (ASTO-Non-standard)
申请日期	2023-11-17
专利号	RU2828103-C1
成果简介	NOVELTY - Present invention relates to molecular cytogenetics. Described is a method of producing a panel of DNA probes for determining chromosomal translocations, deletions and amplifications, involving development of a panel of DNA probes using long ones up to 15 kbp sequences of PCR amplifications with subsequent formation of DNA libraries for diagnosed sections of human genome and control loci, wherein said method includes steps of: selecting target regions of the genome; selection and synthesis of oligonucleotide primers for target regions of the genome; production of DNA products using PCR of long fragments; obtaining a library of DNA fragments using an enzymatic fragmentation method; ligation of the synthesized adapter sequences and amplification of the library using the synthesized target primer sequences; introduction into a genomic library of a fluorescent label; checking the specificity and intensity of the DNA probe in the FISH reaction. USE - Biotechnology. ADVANTAGE - Disclosed method can be used to determine chromosomal translocations, deletions and amplifications based on fluorescent hybridization of a DNA probe with a genome region with a length of more than 30 kbp, in various biological samples of human cells. 1 cl, 1 dwg
IPC 分类号	A61K-048/00 ; C07H-021/04 ; C12Q-001/68
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/19253
专题	中国科学院新疆生态与地理研究所
作者单位	AS RUSSIA TOMSK NAT RES MEDICAL CENT (ASTO-Non-standard)
推荐引用方式 GB/T 7714	VASILYEV S A,ZUEV A S,SAZHENOVA E A,et al. METHOD OF PRODUCING PANEL OF DNA PROBES FOR DETERMINING CHROMOSOMAL TRANSLOCATIONS, DELETIONS AND AMPLIFICATIONS. RU2828103-C1[P]. 2023.

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