| UNIVERSAL INTEGRATION VECTOR PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT HUMAN ANTIBODY TO RECEPTOR-BINDING DOMAIN (RBD) OF CORONAVIRUS SARS-CoV-2 IN MAMMALIAN CELLS OBTAINED USING UNIVERSAL VECTOR & RECOMBINANT MONOCLONAL ANTIBODY DEV_K57, HAVING VIRUS-NEUTRALIZING ACTIVITY ON SARS-CoV-2 | |
| 2023-11-20 | |
| 专利权人 | UNIV URAL FEDERAL (UYUR-C) |
| 申请日期 | 2023-11-20 |
| 专利号 | RU2829359-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, in particular to a universal integration vector SB7G_HIGH for cloning cDNA encoding variable domains of antibodies, comprising an expression cassette flanked by inverted repeats for integration with Sleeping beauty (SB) transposase in the eukaryotic cell genome, as well as to recombinant plasmid SB7G_HIGH_DEV_K57, obtained therefrom. Also disclosed is a recombinant monoclonal antibody DEV_K57, having virus-neutralizing activity on SARS-CoV-2 variants of Delta and Omicron. USE - Biotechnology. ADVANTAGE - Invention is effective for producing genetic structures that provide more efficient synthesis and secretion of recombinant monoclonal antibodies in a eukaryotic producer cell line CHO-S, which is characterized by more effective post-translational modification and higher output of said recombinant antibodies having virus-neutralizing activity on SARS CoV 2 virus of Delta and Omicron variants. 3 cl, 13 dwg, 5 tbl, 5 ex |
| IPC 分类号 | C07K-016/10 ; C12N-015/63 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19224 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV URAL FEDERAL (UYUR-C) |
| 推荐引用方式 GB/T 7714 | SHESTAKOVA A A,DUBROVSKAIA E V,PRUDNIKOVA E YU,et al. UNIVERSAL INTEGRATION VECTOR PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT HUMAN ANTIBODY TO RECEPTOR-BINDING DOMAIN (RBD) OF CORONAVIRUS SARS-CoV-2 IN MAMMALIAN CELLS OBTAINED USING UNIVERSAL VECTOR & RECOMBINANT MONOCLONAL ANTIBODY DEV_K57, HAVING VIRUS-NEUTRALIZING ACTIVITY ON SARS-CoV-2. RU2829359-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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