| METHOD FOR PRODUCING TISSUE-ENGINEERED IN VITRO MODEL OF HUMAN HEAD AND NECK SQUAMOUS CELL CARCINOMA | |
| 2023-12-01 | |
| 专利权人 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 申请日期 | 2023-12-01 |
| 专利号 | RU2825474-C1 |
| 成果简介 | NOVELTY - Invention relates to biotechnology, in particular to a method for producing a tissue-engineered in vitro model of human head and neck squamous cell carcinoma, characterized by that a cell component of the structure, which is cultured in a basic growth medium consisting of DMEM/F12 and 10 % foetal calf serum, an immortalized line of squamous cell carcinoma of head and neck or a primary cell culture obtained from a biopsy material of tumour tissue of squamous cell carcinoma of the head and neck, or a suspension of cells isolated from a biopsy material of tumour tissue of squamous cell carcinoma of head and neck without subsequent expansion ex vivo, is combined with a matrix of the structure, in form of cryoprecipitate obtained from stored at -20 °C blood plasma by its defrosting at 3 °C and concentration of plasma proteins by 5 times after deposition at 5000g, at rate of 200 thousand living cells per 100-300 mcl of cryoprecipitate, incubated for 2 minutes at room temperature, then the total volume of the suspension with the base growth medium is adjusted to 2 ml, which are transferred into well of 24-well plate with an ultra-low adhesive coating, then the plate is placed on an orbital shaker operating at 50 rpm and located in a CO2 incubator providing standard cultivation conditions, namely 37 °C, 5% CO2 and saturated humidity, and cultivated for at least 2 days. USE - Biotechnology. ADVANTAGE - Invention enables to obtain a standardized tissue-engineered in vitro model of tumour tissue capable of prolonged cultivation and can find application in biomedical research and development for searching for new approaches to treating socially significant diseases. 1 cl, 5 dwg, 1 tbl, 3 ex |
| IPC 分类号 | C12N-005/07 ; C12N-005/09 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19108 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 推荐引用方式 GB/T 7714 | FATKHUDINOV T KH,ARUTYUNYAN I V,MAKAROV A V,et al. METHOD FOR PRODUCING TISSUE-ENGINEERED IN VITRO MODEL OF HUMAN HEAD AND NECK SQUAMOUS CELL CARCINOMA. RU2825474-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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