| METHOD FOR DETECTING ISA1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | |
| 2023-12-01 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-12-01 |
| 专利号 | RU2833963-C1 |
| 成果简介 | NOVELTY - Present invention relates to genetic engineering of plants, namely to genetic markers detecting genetic modifications of the ISA1 gene, obtained using CRISPR/Cas9 on genome of durum wheat (Triticum durum) and winter and spring triticale (×Triticosecale). Disclosed is a method based on carrying out a polymerase chain reaction (PCR) with oligonucleotide primers, flanking an intended editing site, with subsequent detection of PCR results using capillary electrophoresis. USE - Biotechnology. ADVANTAGE - Invention enables to detect ISA1 gene editing events in cereals arising from editing using the CRISPR/Cas9 system. 3 cl, 7 dwg, 1 tbl, 2 ex |
| IPC 分类号 | C12N-015/82 ; C12Q-001/6806 ; C12Q-001/686 ; C12Q-001/6876 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19099 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | SAMARINA M A,DIVASHUK M G,KRUPIN P I,et al. METHOD FOR DETECTING ISA1 GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES. RU2833963-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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