METHOD FOR DETERMINING SOURCE OF HIV INFECTION DURING MOLECULAR-EPIDEMIOLOGICAL INVESTIGATIONS USING SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND DEVELOPED REGIONAL DATABASE
2023-12-04
专利权人UNIV URAL FEDERAL (UYUR-C)
申请日期2023-12-04
专利号RU2830591-C1
成果简介NOVELTY - Disclosed is a method for determining the source of HIV infection during molecular-epidemiological investigations. Method involves nested two-step PCR with a set of primers for step 1 hivoutf 5'-gcgactggtgagtacgcc-3', 5'-tcttctggggcttgttcc-3' and for stage 2 hivinf 5'-ccctcaaatcactctttggc-3' and hivinr 5'-ccacacaatcatcacctgcc-3' to obtain a long fragment of the HIV genome, including the gag and pol genes, sequencing and obtaining a sequence of the analyzed fragments, phylogenetic analysis with inclusion of sequences from the database of patients with virologic ineffectiveness of ART in NWFD with subsequent identification of a possible source of infection. USE - Medicine; virology. ADVANTAGE - Invention increases resolution of phylogenetic analysis when determining a possible source of HIV infection. 1 cl, 1 dwg, 5 tbl, 1 ex
IPC 分类号C12Q-001/68
国家俄罗斯
专业领域生物科学
语种英语
成果类型专利
文献类型科技成果
条目标识符http://119.78.100.226:8889/handle/3KE4DYBR/19066
专题中国科学院新疆生态与地理研究所
作者单位
UNIV URAL FEDERAL (UYUR-C)
推荐引用方式
GB/T 7714
SHCHEMELEV A N,SEMENOV A V,OSTANKOVA YU V,et al. METHOD FOR DETERMINING SOURCE OF HIV INFECTION DURING MOLECULAR-EPIDEMIOLOGICAL INVESTIGATIONS USING SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND DEVELOPED REGIONAL DATABASE. RU2830591-C1[P]. 2023.
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