| METHOD OF PRODUCING FINISHED DOSAGE FORM OF PROBIOTIC AGENT BASED ON STRAINS BIFIDOBACTERIUM LONGUM SUBSPECIES LONGUM AND LACTICASEIBACILLUS PARACASEI SUBSPECIESPARACASEI | |
| 2023-12-06 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-12-06 |
| 专利号 | RU2829576-C1 |
| 成果简介 | NOVELTY - Disclosed is a method for producing a finished dosage form of a probiotic agent based on bacterial strains Bifidobacterium longum subsp. longum VKM B-3717D and Lacticaseibacillus paracasei subsp. paracasei VKM B-3711D, including cultivation for 48 hours of bacteria at 37 °C in anaerobic conditions in an atmosphere of a three-component gas mixture 80% N2, 10% CO2, 10% H2; then, under anaerobic conditions, preparing a protective medium consisting of 1 part of a stock solution prepared under anaerobic conditions at 0.05 mg of lacto-N-biose (C14H25NO11) or N-acetylglucosamine per 100 ml in skim milk, and 1 part of sterile skim milk or sucrose-gelatinous medium; bottled in containers – penicillin bottles or ampoules; visible growth of bacteria is removed from the agar surface in each Petri dish with a sterile disposable viscose swab-probe into each container, containers are placed in aseptic sealed containers, then frozen and lyophilized in a given mode. After drying, the contents of each container are homogenised, 30 mg of chitosan, 50 mg of sucrose and 70 mg of lactose are added per 1 therapeutic dose of the preparation, including 7×107 to 8×107 CFU of each bacterial strain used, cotton plugs are replaced with rubber ones; then the bottles are sealed with a metal cap; if the finished dosage form of the probiotic agent is dried in ampoules, the ampoules are sealed or packed into enteric capsules at rate of 1 therapeutic dose per 1 capsule No. 3. USE - Biotechnology. ADVANTAGE - Invention provides stability of the titre of bacterial strains in the composition of the medicinal agent for at least three years of storage in the lyophilised form. 1 cl, 4 tbl, 4 ex |
| IPC 分类号 | A61K-035/745 ; A61K-035/747 ; C12N-001/20 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/19035 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | YUDIN S M,KESKINOV A A,MAKAROV V V,et al. METHOD OF PRODUCING FINISHED DOSAGE FORM OF PROBIOTIC AGENT BASED ON STRAINS BIFIDOBACTERIUM LONGUM SUBSPECIES LONGUM AND LACTICASEIBACILLUS PARACASEI SUBSPECIESPARACASEI. RU2829576-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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