RECOMBINANT STRAIN OF BACTERIA ESCHERICHIA COLI THAT IS PRODUCER OF CHIMERIC PROTEIN FORMAMIDOPYRIMIDINE-DNA-GLYCOSYLASE WITH FLUORESCENT REPORTER PROTEIN
	BULGAKOV N A; DVORNIKOVA A P; PETROVA D V; DMITRY ZH
	2023-12-12
专利权人	UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) ; AS SIBE CHEM BIOLOGY & FUNDAMENTAL MEDIC (ASIC-Non-standard)
申请日期	2023-12-12
专利号	RU2834027-C1
成果简介	NOVELTY - Disclosed is a recombinant bacterial strain Escherichia coli which is a producer of chimeric protein formamidopyrimidine-DNA-glycosylase (FPG) with a fluorescent reporter protein (EGFP). Said strain is obtained by transformation of cells Escherichia coli strain BL21 (DE3) plasmid pET24b(+)-Fpg-(G4S)3-TEVs-EGFP, having a nucleotide sequence SEQ ID NO: 1, constructed on the basis of the pET24b (+) vector and containing the f1 replication origin, the T7 phage promoter, the RBS ribosome entry site, built-in gene coding chimeric recombinant protein FPG and EGFP, having amino acid sequence SEQ ID NO: 2, a phage T7 terminator, a kanamycin resistance gene Kan (R), a lactose repressor lacI and a lactose operator. USE - Biotechnology. ADVANTAGE - Invention enables to obtain a recombinant producer strain which enables to obtain a chimeric recombinant protein formamidopyrimidine-DNA glycosylase (Fpp) with a fluorescent reporter protein (EGFP) capable of forming a DNA-protein cross-link. 1 cl, 8 dwg, 4 ex
IPC 分类号	C07K-019/00 ; C12N-001/21 ; C12N-015/62 ; C12N-015/70
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18972
专题	中国科学院新疆生态与地理研究所
作者单位	1.UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) 2.AS SIBE CHEM BIOLOGY & FUNDAMENTAL MEDIC (ASIC-Non-standard)
推荐引用方式 GB/T 7714	BULGAKOV N A,DVORNIKOVA A P,PETROVA D V,et al. RECOMBINANT STRAIN OF BACTERIA ESCHERICHIA COLI THAT IS PRODUCER OF CHIMERIC PROTEIN FORMAMIDOPYRIMIDINE-DNA-GLYCOSYLASE WITH FLUORESCENT REPORTER PROTEIN. RU2834027-C1[P]. 2023.

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