METHOD OF PRODUCING UNIFIED INTESTINAL MEDIUM FOR CULTIVATION OF INTESTINAL BACTERIA
	SANDULIAN K V; POPOV I V
	2023-12-13
专利权人	SANDULIAN K V (SAND-Individual) ; POPOV I V (POPO-Individual)
申请日期	2023-12-13
专利号	RU2834475-C1
成果简介	NOVELTY - Invention relates to biotechnology. Disclosed is a method of producing a unified intestinal medium for culturing intestinal bacteria for medical and agricultural microbiology, comprising adding to 1 l of distilled water 9.0 g of pectin, 9.0 g of xylan, 9.0 g of arabinogalactan, 9.0 g of amylopectin, 43.7 g of casein, 74.6 g of starch, 31.5 g of Tween 80, 43.7 g of bacto peptone, 0.7 g of sterile bovine bile, 4.7 g of K2HPO4x3H2O, 8.4 g of NaCl, 0.009 g of FeSO4x7H2O, 0.7 g of MgSO4x7H2O, 0.8 g of CaCl2x2H2O, 0.02 g of hemin, 0.2 g of p-aminobenzoic acid. Obtained mixture is autoclaved for 30 minutes at 134 °C and pressure of 2 atm, then 2.5 g of nicotinamide, 0.004 g of thiamine, 0.003 g of cysteine HCl are added⋅. If the unified intestinal medium is used for more than 72 hours, 1.5 g of the vitamin mixture containing per 1 l: 0.001 g of menadione, 0.002 g of D-biotin, 0.0005 g of vitamin B12, 0.01 g of pantothenic acid, 0.004 g of thiamine, 0.005 g of nicotinamide acid. USE - Biotechnology. ADVANTAGE - Invention provides cultivation of the intestinal microbiota, which is not cultivated in standard intestinal media, and reproduction of the microbiota composition for a long time of cultivation. 1 cl, 6 dwg
IPC 分类号	C12N-001/20 ; C12Q-001/02 ; G09B-023/28
国家	俄罗斯
专业领域	生物科学
语种	英语
成果类型	专利
文献类型	科技成果
条目标识符	http://119.78.100.226:8889/handle/3KE4DYBR/18943
专题	中国科学院新疆生态与地理研究所
作者单位	1.SANDULIAN K V (SAND-Individual) 2.POPOV I V (POPO-Individual)
推荐引用方式 GB/T 7714	SANDULIAN K V,POPOV I V. METHOD OF PRODUCING UNIFIED INTESTINAL MEDIUM FOR CULTIVATION OF INTESTINAL BACTERIA. RU2834475-C1[P]. 2023.

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