| METHOD OF PRODUCING SELF-ASSEMBLED OCTAMERIC PROTEIN COMPLEX BASED ON APOFERRITIN | |
| 2023-12-15 | |
| 专利权人 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 申请日期 | 2023-12-15 |
| 专利号 | RU2834642-C1 |
| 成果简介 | NOVELTY - Invention relates to chemistry and biotechnology, specifically to a method of producing a recombinant protein complex based on apoferritin in an octameric state, in which apoferritin acts as a carrier for multiple presentation of another protein, according to which recombinant strain-producer E. coli containing hybrid plasmid containing under control of rhamnose promoter nucleotide sequence of fused genes of presented protein is cultivated and apoferritin, coding a monomer of a protein complex, on a nutrient medium containing yeast extract, bacto tryptone, glycerol, potassium phosphate buffer and MgSO4, which during preparation of medium are autoclaved together, then lysate is obtained from the cells obtained during culturing, which is centrifuged, filtering, followed by adding imidazole to obtain a solution from which the protein complex is isolated by nickel-affinity chromatography. USE - Chemistry; biotechnology. ADVANTAGE - Biosynthesis, isolation and purification of a recombinant protein complex based on apoferritin in an octameric state, in which apoferritin acts as a carrier for multiple presentation of another protein. 1 cl, 4 dwg, 1 tbl, 3 ex |
| IPC 分类号 | C07K-001/22 ; C07K-014/00 ; C07K-019/00 ; C12N-015/70 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18902 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | UNIV MISIS NAT SCI & TECHNOLOGY (UMNS-C) |
| 推荐引用方式 GB/T 7714 | VLASOV A V,SUDAREV V V,BAZHENOV S V,et al. METHOD OF PRODUCING SELF-ASSEMBLED OCTAMERIC PROTEIN COMPLEX BASED ON APOFERRITIN. RU2834642-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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