| IMMUNOLIPOSOME WHICH BINDS TO RBD OF S-PROTEIN SARS-CoV-2 | |
| 2023-12-26 | |
| 专利权人 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 申请日期 | 2023-12-26 |
| 专利号 | RU2827165-C1 |
| 成果简介 | NOVELTY - Described is an immunoliposome which binds to RBD of S-protein SARS-CoV-2, containing barnase in its inner volume, the outer surface of which is covalently modified with a monoclonal neutralizing antibody P4A1-2A. Also disclosed is a method of producing an immunoliposome, comprising: mixing a barnase solution with a phospholipid suspension, 7-fold freezing/thawing, forcing through an extruder using polycarbonate filters with a pore diameter of 100 nm, gel-permeable chromatography on a column with a sepharose sorbent CL-2B with subsequent modification for 40 minutes at room temperature of the outer surface of liposome with 2-iminothiolane to obtain a thiolated liposome, introducing a maleimide group into the P4A1-2A antibody by mixing with 100-fold molar excess of the sulfo-EMCS reagent and incubating for 40 minutes at room temperature, mixing the thiolated liposome containing barnase in the inner volume, with P4A1- 2A antibody containing maleimide groups, and incubation for 1 hour at room temperature to obtain an immunoliposome that binds to RBD of the S-protein SARS-CoV-2. USE - Biotechnology; medicine. ADVANTAGE - Increased neutralizing activity of the P4A1-2A antibody. 4 cl, 6 dwg, 7 ex |
| IPC 分类号 | A61K-039/395 |
| 国家 | 俄罗斯 |
| 专业领域 | 生物科学 |
| 语种 | 英语 |
| 成果类型 | 专利 |
| 文献类型 | 科技成果 |
| 条目标识符 | http://119.78.100.226:8889/handle/3KE4DYBR/18725 |
| 专题 | 中国科学院新疆生态与地理研究所 |
| 作者单位 | FEDERAL STATE BUDGETARY INST (FSBI-C) |
| 推荐引用方式 GB/T 7714 | BOBIK T V,SIMONOVA M A,RUSHKEVICH N YU,et al. IMMUNOLIPOSOME WHICH BINDS TO RBD OF S-PROTEIN SARS-CoV-2. RU2827165-C1[P]. 2023. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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